Molecular characterization of the genotypic diversity, resistance profile and pathogenic potential of Salmonella Infantis strains isolated from multiple sources in Brazil

• Grant number: 19/06947-6
• Support Opportunities: Scholarships in Brazil - Master
• Start date until: July 01, 2019
• End date until: February 28, 2021
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Principal Investigator: Juliana Pfrimer Falcão
• Grantee: Felipe Pinheiro Vilela
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated scholarship(s): 19/24815-0 - Whole-genome sequencing and phylogenetic analysis of Salmonella infantis strains isolated from multiple sources in Brazil, BE.EP.MS

Abstract

Salmonellosis caused by non-typhoidal serovars is among one of the most reported foodborne infection worldwide. Strains of Salmonella Infantis (S. Infantis), a non-specific host serovar, are capable to infect multiple animal reservoirs besides humans and have been reported presenting multidrug resistant profiles. This project aims to verify the genetic diversity of S. Infantis strains isolated from multiple sources in Brazil and also to determine the pathogenic potential and the phenotypic and genotypic antimicrobial resistance profiles to. For that, 149 S. Infantis strains isolated from humans, animals, food and the environment in Brazil between 2013 and 2018 will be molecularly typed by Pulsed-field gel electrophoresis (PFGE) and, based on the whole-genome sequencing (WGS) of these strains it will be typed by Single-Nucleotide Polymorphisms (SNPs), Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and core genome Multilocus Sequencing Type (cgMLST). The presence of 16 virulence genes related to Salmonella pathogenicity islands (SPI-1 and SPI-2) will also be verified through data generated by WGS. Moreover, the antimicrobial susceptibility profile will be determined against 18 antimicrobial agents by the disk-diffusion test, as well as the presence of some related resistance genes and quinolone resistance determining region (QRDRs) mutations of all the strains will be searched. The results to be obtained should contribute to a better characterization of the circulating S. Infantis strains in Brazil in recent years, as well as elucidate possible characteristics and/or genotypic profiles to provide a better understanding of the infection and disease caused by this serovar of great clinical importance. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VILELA, FELIPE PINHEIRO; RODRIGUES, DALIA DOS PRAZERES; ALLARD, MARC WILLIAM; FALCAO, JULIANA PFRIMER. Genomic characterization and antimicrobial resistance profiles of Salmonella enterica serovar Infantis isolated from food, humans and veterinary-related sources in Brazil. Journal of Applied Microbiology, JAN 2022. (19/19338-8, 19/06947-6)

VILELA, FELIPE PINHEIRO; RODRIGUES, DALIA DOS PRAZERES; COSTA, RENATA GARCIA; TIBA CASAS, MONIQUE RIBEIRO; FALCAO, JULIANA PFRIMER; CAMPIONI, FABIO. High similarity and high frequency of virulence genes among Salmonella Dublin strains isolated over a 33-year period in Brazil. Brazilian Journal of Microbiology, v. 51, n. 2, NOV 2019. (17/05756-7, 19/06947-6, 13/25191-3, 16/24716-3)

VILELA, FELIPE PINHEIRO; RODRIGUES, DALIA DOS PRAZERES; FERREIRA, JOSEANE CRISTINA; DA COSTA DARINI, ANA LUCIA; ALLARD, MARC WILLIAM; FALCAO, JULIANA PFRIMER. Genomic characterization of Salmonella enterica serovar Choleraesuis from Brazil reveals a swine gallbladder isolate harboring colistin resistance gene mcr-1.1. Brazilian Journal of Microbiology, v. 53, n. 4, p. 8-pg., 2022-08-19. (19/19338-8, 19/06947-6)

VILELA, FELIPE PINHEIRO; PRIBUL, BRUNO ROCHA; RODRIGUES, DALIA DOS PRAZERES; BALKEY, MARIA; ALLARD, MARC; FALCAO, JULIANA PFRIMER. Draft Genome Sequences of 80 Salmonella enterica Serovar Infantis Strains Isolated from Food, Environmental, Human, and Veterinary Sources in Brazil. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 10, n. 24, JUN 2021. (19/06947-6, 19/19338-8)

VILELA, FELIPE PINHEIRO; GOMES, CAROLINA NOGUEIRA; PAZIANI, MARIO HENRIQUE; BRAZ, VANIA SANTOS; RODRIGUES, DALIA DOS PRAZERES; COSTA, RENATA GARCIA; TIBA-CASAS, MONIQUE RIBEIRO; VON ZESKA KRESS, MARCIA REGINA; FALCAO, JULIANA PFRIMER; CAMPIONI, FABIO. Virulence traits and expression of bstA, fliC and sopE2 in Salmonella Dublin strains isolated from humans and animals in Brazil. INFECTION GENETICS AND EVOLUTION, v. 80, JUN 2020. (13/25191-3, 17/05756-7, 19/06947-6, 16/24716-3)

VILELA, FELIPE PINHEIRO; FALCAO, JULIANA PFRIMER; CAMPIONI, FABIO. Analysis of Resistance Gene Prevalence in Whole-Genome Sequenced Enterobacteriales from Brazil. MICROBIAL DRUG RESISTANCE, v. 26, n. 6, NOV 2019. (19/06947-6, 16/24716-3)

VILELA, FELIPE PINHEIRO; FALCAO, JULIANA PFRIMER. Analysis of the antimicrobial resistance gene frequency in whole-genome sequenced Vibrio from Latin American countries. Journal of Medical Microbiology, v. 70, n. 9, 2021. (19/06947-6, 19/19338-8)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

VILELA, Felipe Pinheiro. Molecular characterization of the genotypic diversity, resistance profile and pathogenic potential of Salmonella Infantis strains isolated from multiple sources in Brazil. 2021. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Ciências Farmacêuticas de Ribeirão Preto (PCARP/BC) Ribeirão Preto.