Comparative analysis of the genome, transcriptome and phenotypic characterization of Salmonella typhimurium strains isolated from humans and food in Brazil

• Grant number: 17/06633-6
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: April 01, 2018
• End date: March 31, 2021
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Juliana Pfrimer Falcão
• Grantee: Amanda Aparecida Seribelli
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium) is one of the leading causes of gastroenteritis worldwide. Approximately 93.8 million cases of salmonellosis occur annually by non-typhoid Salmonella around the world. However, in Brazil there are few studies that have elucidated possible differences in the pathogenicity, virulence and genotypic diversity of S. Typhimurium strains isolated from humans and food. This project aims to compare the genome, to analyze the transcriptome and the phenotypic profile of S. Typhimurium strains isolated from humans and food during 31 years in different regions in Brazil. The comparative genome analysis will be performed for 92 strains isolated from humans (43) and food (49). For 40 strains isolated from humans (20) and food (20), it will be performed the phenotypic tests of epithelial cell invasion (CACO-2), macrophages survival test (J774), acid and oxidative stress tolerance tests. Moreover, the transcriptomes analysis of two S. Typhimurium strains will be performed, the RNAs will be extracted after the tests of tolerance to acid and oxidative stress and under ideal growth conditions for such strains. In addition, the median lethal dose (LD50) of two S. Typhimurium strains selected will be determined. It should be noted that there are no published studies comparing the S. Typhimurium strains isolated during different periods and sources in the country. There are no published data that gathered the analysis of the complete genomes, transcriptomes and phenotypic tests mentioned above. Therefore, the results to be obtained will probably contribute for a better characterization of the pathogenicity, virulence and genotypic diversity of this global important enteropathogen. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SERIBELLI, AMANDA AP.; DA SILVA, PATRICK; DA CRUZ, MARCELO FERREIRA; DE ALMEIDA, FERNANDA; FRAZAO, MILIANE R.; MEDEIROS, MARTA I. C.; RODRIGUES, DALIA DOS P.; KICH, JALUSA D.; DE JESUS BENEVIDES, LEANDRO; SOARES, SIOMAR DE C.; et al. nsights about the epidemiology of Salmonella Typhimurium isolates from different sources in Brazil using comparative genomic. GUT PATHOGENS, v. 13, n. 1, APR 28 2021. (16/24716-3, 19/19338-8, 17/06633-6)

ALMEIDA, FERNANDA; SERIBELLI, AMANDA APARECIDA; CAZENTINI MEDEIROS, MARTA INES; RODRIGUES, DALIA DOS PRAZERES; DE MELLOVARANI, ALESSANDRO; LUO, YAN; ALLARD, MARC W.; FALCAO, JULIANA PFRIMER. Phylogenetic and antimicrobial resistance gene analysis of Salmonella Typhimurium strains isolated in Brazil by whole genome sequencing. PLoS One, v. 13, n. 8, AUG 13 2018. (12/19132-1, 17/06633-6)

SERIBELLI, AMANDA APARECIDA; CRUZ, MARCELO FERREIRA; VILELA, FELIPE PINHEIRO; FRAZAO, MILIANE RODRIGUES; PAZIANI, MARIO H.; ALMEIDA, FERNANDA; CAZENTINI MEDEIROS, MARTA INES; RODRIGUES, DALIA DOS PRAZERES; VON ZESKA KRESS, MARCIA R.; ALLARD, MARC W.; et al. Phenotypic and genotypic characterization of Salmonella Typhimurium isolates from humans and foods in Brazil. PLoS One, v. 15, n. 8, AUG 18 2020. (16/24716-3, 17/06633-6)

SERIBELLI, AMANDA AP.; DA SILVA, PATRICK; DA CRUZ, MARCELO FERREIRA; DE ALMEIDA, FERNANDA; FRAZAO, MILIANE R.; MEDEIROS, MARTA I. C.; RODRIGUES, DALIA DOS P.; KICH, JALUSA D.; DE JESUS BENEVIDES, LEANDRO; SOARES, SIOMAR DE C.; et al. Insights about the epidemiology of Salmonella Typhimurium isolates from different sources in Brazil using comparative genomics. GUT PATHOGENS, v. 13, n. 1, p. 15-pg., 2021-04-28. (17/06633-6, 16/24716-3, 19/19338-8)

SERIBELLI, AMANDA APARECIDA; MACHADO RIBEIRO, TAMARA R.; DA SILVA, PATRICK; MARTINS, ISABELA MANCINI; VILELA, FELIPE PINHEIRO; CAZENTINI MEDEIROS, I, MARTA; PERONNI, KAMILA CHAGAS; DA SILVA JUNIOR, WILSON ARAUJO; MOREIRA, CRISTIANO GALLINA; FALCAO, JULIANA PFRIMER. Salmonella Typhimurium ST313 isolated in Brazil revealed to be more invasive and inflammatory in murine colon compared to ST19 strains. JOURNAL OF MICROBIOLOGY, v. 59, n. 9, AUG 2021. (17/06633-6, 16/12744-2, 19/19338-8, 19/03049-7, 16/24716-3)

SERIBELLI, AMANDA APARECIDA; DA SILVA, PATRICK; FRAZAO, MILIANE RODRIGUES; KICH, JALUSA DEON; ALLARD, MARC W.; FALCAO, JULIANA PFRIMER. Phylogenetic relationship and genomic characterization of Salmonella Typhimurium strains isolated from swine in Brazil. INFECTION GENETICS AND EVOLUTION, v. 93, SEP 2021. (16/24716-3, 17/06633-6, 19/19338-8)

AP SERIBELLI, AMANDA; GONZALES, JULIA C.; DE ALMEIDA, FERNANDA; BENEVIDES, LEANDRO; CAZENTINI MEDEIROS, I, MARTA; RODRIGUES, DALIA DOS PRAZERES; SOARES, SIOMAR DE C.; ALLARD, MARC W.; FALCAO, JULIANA P.. Phylogenetic analysis revealed that Salmonella Typhimurium ST313 isolated from humans and food in Brazil presented a high genomic similarity. Brazilian Journal of Microbiology, v. 51, n. 1, p. 53-64, MAR 2020. (16/24716-3, 17/06633-6)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SERIBELLI, Amanda Aparecida. Analyses of the genomes, transcriptomes and phenotypic characterization of Salmonella Typhimurium strains isolated from humans, food and swine in Brazil. 2021. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Ciências Farmacêuticas de Ribeirão Preto (PCARP/BC) Ribeirão Preto.