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Biotechnological potential and phenotypic and molecular characterization of Salmonella enterica mutants

Grant number: 17/10051-2
Support type:Regular Research Grants
Duration: October 01, 2017 - May 31, 2020
Field of knowledge:Biological Sciences - Microbiology - Applied Microbiology
Principal Investigator:Marcelo Brocchi
Grantee:Marcelo Brocchi
Home Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Previous results from our group indicated that null-mutants of Salmonella enterica for genes encoding Naps (Nucleoid-associated proteins) are attenuated and able to induce protection in the murine model. Data also indicated that some strains presented antitumor properties. Altogether, those results indicate an important biological role of NAPs in S. enterica. Therefore, the general objective of this project is to further investigate the biological roles of some NAPs in S. enterica and also to explore the biotechnological potential attenuated Salmonella strains as vaccines. The specific objectives are: (1) Elucidate the biological role of the NAP YbaB in S. enterica Typhimurium by phenotypic, gene expression and transcriptome analyses; (2) Characterize a strain of S. enterica Typhimurium DstpA regarding the attenuation of virulence in the murine model and a possible role of this Nap in biofilm formation; (3) Characterize the IHF (Integration-host factor) mutants of S. enterica Typhimurium regarding colonization and immunogenicity in avian; (4) Characterize the IHF (Integration-host factor) mutants of S. enterica Typhimurium regarding antitumoral activity in the murine model; (5) Construction of a recombinant attenuated S. enterica strain (RASV) carrying a DNA vaccine for ZIKV virus. This Project in intended to be conducted in collaboration with groups abroad. (AU)