Phenotypic and molecular characterization of attenuated strains of Salmonella enterica Typhimurium

• Grant number: 13/05590-0
• Support Opportunities: Scholarships in Brazil - Master
• Start date: June 01, 2013
• End date: April 30, 2015
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Principal Investigator: Marcelo Brocchi
• Grantee: Meiriele da Silva das Neves
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Recent studies of our group have indicated that null mutants of Salmonella enterica to ihfA or ihfB, genes responsible to encode subunits A and B of IHF (Integration host factor), respectively, show virulence attenuation and are capable of inducing protection in a murine model of S. enterica infection. Based on these results, the objective of this project is to further investigate the biological role of IHF in S. enterica Typhimurium. This will be achieved by characterization of mutant strains ”ihfA or ”ihfB for phenotyes such as growth in vitro, motility, survival in stationary phase, under acidic conditions, in the presence of bile salts and on the invasiveness and survival in macrophages. The immunogenicity of the mutants will be evaluated using the murine model of infection. In addition, gene expression of ihfA and ihfB in two strains of S. enterica will be evaluated by real-time PCR. Such studies will further evaluate the role of IHF in the virulence of S. enterica Typhimurium and the potential of these strains to be used as a live vaccine strain.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

NEVES, Meiriele da Silva das. Phenotipic and molecular characterization of attenuated strains of Salmonella enterica Typhimurium. 2015. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia Campinas, SP.