Phenotypic and genotypic comparison of Salmonella Dublin strains isolated from humans and animals in Brazil

• Grant number: 15/10818-6
• Support type: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): July 01, 2015
• Effective date (End): December 31, 2016
• Field of knowledge: Biological Sciences - Microbiology
• Principal Investigator: Fábio Campioni
• Grantee: Felipe Pinheiro Vilela
• Home Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Salmonellosis by nontyphoidal serovars is among the most common food-borne infections worldwide. Salmonella Dublin is a serovar strongly adapted to cattle causing enteritis and/or systemic disease with high rates of mortality. However, sporadically it can be isolated from humans, usually causing serious disease, specially in patients with underlying chronic diseases. This project aims to analyze comparatively S. Dublin strains isolated from animals and humans using molecular typing methods and some phenotypic and genotypic tests related to virulence. Also, the resistance of those strains to antimicrobials will be verified. For this, 60 S. Dublin strains isolated from humans and animals in Brazil will be molecularly typed by Pulsed-field gel electrophoresis (PFGE) and Multiple-locus variable-number tandem repeat analysis (MLVA) to acess theis genetic diversity. Moreover, the expression of the capsular antigen Vi will be verified by serological test and searching for the presence of tviB gene by PCR. Furthermore, the presence of flagella will be verified by the motility test and the presence of the virulence plasmid pSDL2 will be verified by PCR. The susceptibility profile will be verified by the disc diffusion technique using 16 antimicrobials. The results to be obtained will probably contribute for a better characterization of S. Dublin, as well as, may elucidate possible characteristics and/or genotypic traits that contribute for this microorganism, strongly adapted to cattle, to cause serious disease in humans.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VILELA, FELIPE PINHEIRO; GOMES, CAROLINA NOGUEIRA; PASSAGLIA, JAQUELINE; RODRIGUES, DALIA PRAZERES; COSTA, RENATA GARCIA; TIBA CASAS, MONIQUE RIBEIRO; FERNANDES, SUELI APARECIDA; FALCAO, JULIANA PFRIMER; CAMPIONI, FABIO. Genotypic Resistance to Quinolone and Tetracycline in Salmonella Dublin Strains Isolated from Humans and Animals in Brazil. MICROBIAL DRUG RESISTANCE, v. 25, n. 2 SEP 15 2018. Web of Science Citations: 2.

CAMPIONI, FABIO; VILELA, FELIPE PINHEIRO; CAO, GUOJIE; KASTANIS, GEORGE; MILLER, DANIELA; LEON, MARIA SANCHEZ; TIBA-CASAS, MONIQUE RIBEIRO; FERNANDES, SUELI APARECIDA; RODRIGUES, DANA DOS PRAZERES; COSTA, RENATA GARCIA; ALLARD, MARC WILLIAM; FALCAO, JULIANA PFRIMER. Draft Genome Sequences of 112 Salmonella enterica Serovar Dublin Strains Isolated from Humans and Animals in Brazil. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 6, n. 24 JUN 2018. Web of Science Citations: 1.

VILELA, F. P.; FRAZAO, M. R.; RODRIGUES, D. P.; COSTA, R. G.; CASAS, M. R. T.; FERNANDES, S. A.; FALCAO, J. P.; CAMPIONI, F. Genetic diversity, anti-microbial resistance, plasmid profile and frequency of the Vi antigen in Salmonella Dublin strains isolated in Brazil. ZOONOSES AND PUBLIC HEALTH, v. 65, n. 1, p. E34-E43, FEB 2018. Web of Science Citations: 2.