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Study of resistance, virulence and epidemiological profile of Escherichia coli isolated from environment

Grant number: 18/01890-3
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): August 01, 2019
Effective date (End): February 28, 2021
Field of knowledge:Biological Sciences - Microbiology
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Eliana Guedes Stehling
Grantee:João Pedro Rueda Furlan
Home Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Bacterial resistance is a frequent problem in the hospital environment. Increased resistance among members of the Enterobacteriaceae family has resulted in the emergence of multidrug-resistant species, which represent an important public health problem. Among the enterobacteria, the most commonly reported in hospital infections are Escherichia coli, which belongs to the commensal microbiota and may act as an opportunistic pathogen, especially in immunocompromised patients. Different classes of antimicrobials can be used to treat infections caused by this pathogen, including the ²-lactams, quinolones and fluoroquinolones, tetracyclines, aminoglycosides, folate pathway inhibitors, phenicols and polymyxins. Several virulence factors are correlated to the pathogenicity of this bacterium, including toxins and hemolysins. The objective of this research project is to characterize 100 Escherichia coli isolates obtained from the environment regarding antimicrobial resistance, virulence, clonal relationship and epidemiology. Among the 100 isolates, 64 isolates were classified as Multidrug-Resistant (MDR), presenting non-susceptibility mainly to ampicillin (89%), streptomycin (75%), cefazoline and cefuroxime (67%), trimethoprim + sulfamethoxazole, sulfonamide and trimethoprim (52%). Seven isolates have already been characterized for resistance genes, plasmid typing and virulence. The resistance genes blaCMY, blaOXA-1-like, blaSHV, tetA, tetB were detected in all isolates, followed by the sul1 (4), sul2 (3), aadA (3), sul2, aac(6 ')-Ib (2) and floR (1). According to the plasmid typing, IncF, IncY and ColE-like were detected in all isolates, followed by IncFIB (6), IncFIA (1), IncFIC (1), IncHI1 (1) and IncN (1). Among the virulence genes, the ipaH gene was the most prevalent, being detected in four isolates, followed by the stx2 gene (n = 1). Thus, these isolates were classified into two different pathotypes, being EIEC and STEC, respectively. Two isolates did not present any of the virulence genes investigated. MLST analysis was performed on six of the seven previously characterized isolates, and six different STs (ST10/CC10, ST942, ST1684, ST2600, ST6658 and ST8067) were detected. Two isolates from the soil were characterized by the whole genome sequencing, which presented several genes of resistance, virulence and plasmids. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE MELO, FERNANDA MACIEL; RUEDA FURLAN, JOAO PEDRO; SANCHEZ, DANILO GARCIA; LAGE GALLO, INARA FERNANDA; SAVAZZI, EDUARDO ANGELINO; STEHLING, ELIANA GUEDES. Dispersion of merA and catabolic genes in Brazilian water sources. ECOLOGICAL INDICATORS, v. 108, JAN 2020. Web of Science Citations: 0.
RUEDA FURLAN, JOAO PEDRO; LAGE GALLO, INARA FERNANDA; DE CAMPOS, TATIANA AMABILE; STEHLING, ELIANA GUEDES. Genomic Characterization of a Multidrug-Resistant and Hypermucoviscous/Hypervirulent Klebsiella quasipneumoniae subsp. similipneumoniae ST4417 Isolated from a Sewage Treatment Plant. MICROBIAL DRUG RESISTANCE, JAN 2020. Web of Science Citations: 0.
RUEDA FURLAN, JOAO PEDRO; GONCALVES DE ALMEIDA, OTAVIO GUILHERME; PEREIRA DE MARTINIS, ELAINE CRISTINA; STEHLING, ELIANA GUEDES. Characterization of an Environmental Multidrug-Resistant Acinetobacter seifertii and Comparative Genomic Analysis Reveals Co-occurrence of Antimicrobial Resistance and Metal Tolerance Determinants. FRONTIERS IN MICROBIOLOGY, v. 10, SEP 18 2019. Web of Science Citations: 0.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.