Molecular characterization of Escherichia coli isolates from the environment

• Grant number: 18/19539-0
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2019
• End date: July 31, 2021
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Eliana Guedes Stehling
• Grantee: Eliana Guedes Stehling
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Bacterial resistance is a frequent problem in the hospital environment. Increased resistance among members of the Enterobacteriaceae family has resulted in the emergence of multiresistant species, which represent a major public health problem. Among the enterobacteria most commonly reported in hospital infections are Escherichia coli, which belongs to the commensal microbiota and may act as an opportunistic pathogen, especially in immunocompromised patients. Different classes of antimicrobials can be used to treat infections caused by this pathogen, including the classes of ²-lactams, quinolones and fluoroquinolones, tetracyclines, aminoglycosides, folate and polymyxin inhibitors. Several virulence factors are correlated to the pathogenicity of this bacterium, including toxins and hemolysins. The objective of this research project is to characterize 50 isolates of Escherichia coli obtained from the environment regarding antimicrobial resistance, virulence, clonal relation and epidemiology. Sequencing of the complete genome of the isolates with the greatest potential for resistance and virulence and / or isolates that present a phenotypic potential of resistance and do not present the genes will allow the detection of new genes associated with this phenotype or genes that are poorly reported. (AU)

Scientific publications

(26)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE MELO, FERNANDA MACIEL; RUEDA FURLAN, JOAO PEDRO; SANCHEZ, DANILO GARCIA; LAGE GALLO, INARA FERNANDA; SAVAZZI, EDUARDO ANGELINO; STEHLING, ELIANA GUEDES. Dispersion of merA and catabolic genes in Brazilian water sources. ECOLOGICAL INDICATORS, v. 108, JAN 2020. (18/01890-3, 18/19539-0)

RUEDA FURLAN, JOAO PEDRO; SAVAZZI, EDUARDO ANGELINO; STEHLING, ELIANA GUEDES. Genomic insights into multidrug-resistant and hypervirulent Klebsiella pneumoniae co-harboring metal resistance genes in aquatic environments. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, v. 201, SEP 15 2020. (18/19539-0)

FURLAN, PEDRO RUEDA; STEHLING, ELIANA GUEDES. Multiple sequence types, virulence determinants and antimicrobial resistance genes in multidrug- and colistin-resistant Escherichia coli from agricultural and non-agricultural soils. Environmental Pollution, v. 288, NOV 1 2021. (18/19539-0)

RUEDA FURLAN, JOAO PEDRO; LAGE GALLO, INARA FERNANDA; STEHLING, ELIANA GUEDES. enomic characterization of multidrug-resistant extraintestinal pathogenic Escherichia coli isolated from grain culture soil. Pedosphere, v. 32, n. 3, p. 495-502, JUN 2022. (18/19539-0, 18/01890-3)

RUEDA FURLAN, JOAO PEDRO; LOPES, RALF; RAMOS, MICAELA SANTANA; RODRIGUES DOS SANTOS, LUCAS DAVID; ROSA, RAFAEL DA SILVA; SAVAZZI, EDUARDO ANGELINO; STEHLING, ELIANA GUEDES. Colistin-resistant mcr-1-positive Escherichia coli ST1775-H137 co-harboring bla(CTX-M-2) and bla(CMY-2) recovered from an urban stream. INFECTION GENETICS AND EVOLUTION, v. 96, DEC 2021. (18/01890-3, 18/19539-0)

FURLAN, JOAO PEDRO RUEDA; DA SILVA FERREIRA, MARCIA ELIANA; STEHLING, ELIANA GUEDES. Genetic Diversity of Multidrug-Resistant CMY-Producing Escherichia coli from Feces and Soil in a Small-Scale Pig Farm. MICROBIAL DRUG RESISTANCE, v. 26, n. 11, MAY 2020. (18/19539-0)

RAMOS, MICAELA SANTANA; RUEDA FURLAN, JOAO PEDRO; LAGE GALLO, INARA FERNANDA; RODRIGUES DOS SANTOS, LUCAS DAVID; DE CAMPOS, TATIANA AMABILE; SAVAZZI, EDUARDO ANGELINO; STEHLING, ELIANA GUEDES. High Level of Resistance to Antimicrobials and Heavy Metals in Multidrug-Resistant Pseudomonas sp. Isolated from Water Sources. Current Microbiology, v. 77, n. 10, MAY 2020. (18/01890-3, 18/19539-0)

RUEDA FURLAN, JOAO PEDRO; RODRIGUES DOS SANTOS, LUCAS DAVID; SILVA MORETTO, JESSICA APARECIDA; RAMOS, MICAELA SANTANA; LAGE GALLO, INARA FERNANDA; DIAS ALVES, GEORGIA DE ASSIS; PAULELLI, ANA CAROLINA; DE SOUZA ROCHA, CECILIA CRISTINA; CESILA, CIBELE APARECIDA; GALLIMBERTI, MATHEUS; et al. Occurrence and abundance of clinically relevant antimicrobial resistance genes in environmental samples after the Brumadinho dam disaster, Brazil. Science of The Total Environment, v. 726, JUL 15 2020. (18/24069-3, 18/01890-3, 18/19539-0)

FURLAN, JOAO PEDRO RUEDA; LOPES, RALF; STEHLING, ELIANA GUEDES. Multidrug resistance IncC plasmid carrying bla(CMY-97) in Shiga toxin-producing Escherichia coli ST215-H54 of ovine origin. INFECTION GENETICS AND EVOLUTION, v. 93, SEP 2021. (18/19539-0, 18/01890-3)

RUEDA FURLAN, JOAO PEDRO; LOPES, RALF; LIMA GONZALEZ, IRYS HANY; RAMOS, PATRICIA LOCOSQUE; VON ZESKA KRESS, MARCIA REGINA; STEHLING, ELIANA GUEDES. Hypermucoviscous/hypervirulent and extensively drug-resistant QnrB2-, QnrS1-, and CTX-M-3-coproducing Klebsiella pneumoniae ST2121 isolated from an infected elephant (Loxodonta africana). Veterinary Microbiology, v. 251, DEC 2020. (18/19539-0)

RUEDA FURLAN, JOAO PEDRO; MOURA, QUEZIA; LIMA GONZALEZ, IRYS HANY; RAMOS, PATRICIA LOCOSQUE; LINCOPAN, NILTON; STEHLING, ELIANA GUEDES. Draft genome sequence of a multidrug-resistant CTX-M-65-producing Escherichia coli ST156 colonizing a giant anteater (Myrmecophaga tridactyla) in a Zoo. JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, v. 17, p. 2-pg., 2019-06-01. (16/08593-9, 18/19539-0)

RUEDA FURLAN, JOAO PEDRO; LAGE GALLO, INARA FERNANDA; PIRES DE CAMPOS, ANNA CAROLINA LEONELLI; PASSAGLIA, JAQUELINE; FALCAO, JULIANA PFRIMER; NAVARRO, ARMANDO; NAKAZATO, GERSON; STEHLING, ELIANA GUEDES. Molecular characterization of multidrug-resistant Shiga toxin-producing Escherichia coli harboring antimicrobial resistance genes obtained from a farmhouse. PATHOGENS AND GLOBAL HEALTH, NOV 2019. (18/19539-0)

DOS SANTOS, L. D. R.; FURLAN, J. P. R.; GALLO, I. F. L.; RAMOS, M. S.; SAVAZZI, E. A.; STEHLING, E. G.. Occurrence of multidrug-resistant Enterococcus faecium isolated from environmental samples. Letters in Applied Microbiology, v. 73, n. 2, p. 237-246, AUG 2021. (18/01890-3, 18/19539-0)

RUEDA FURLAN, JOAO PEDRO; GONCALVES DE ALMEIDA, OTAVIO GUILHERME; PEREIRA DE MARTINIS, ELAINE CRISTINA; STEHLING, ELIANA GUEDES. Characterization of an Environmental Multidrug-Resistant Acinetobacter seifertii and Comparative Genomic Analysis Reveals Co-occurrence of Antimicrobial Resistance and Metal Tolerance Determinants. FRONTIERS IN MICROBIOLOGY, v. 10, SEP 18 2019. (18/01890-3, 18/19539-0, 17/13759-6)

RODRIGUES DOS SANTOS, LUCAS DAVID; RUEDA FURLAN, JOAO PEDRO; RAMOS, MICAELA SANTANA; LAGE GALLO, INARA FERNANDA; PEREIRA DE FREITAS, LUCAS VICTOR; STEHLING, ELIANA GUEDES. Co-occurrence of mcr-1, mcr-3, mcr-7 and clinically relevant antimicrobial resistance genes in environmental and fecal samples. Archives of Microbiology, v. 202, n. 7, MAY 2020. (18/19539-0)

RUEDA FURLAN, JOAO PEDRO; LAGE GALLO, INARA FERNANDA; DE CAMPOS, TATIANA AMABILE; STEHLING, ELIANA GUEDES. Genomic Characterization of a Multidrug-Resistant and Hypermucoviscous/Hypervirulent Klebsiella quasipneumoniae subsp. similipneumoniae ST4417 Isolated from a Sewage Treatment Plant. MICROBIAL DRUG RESISTANCE, v. 26, n. 11, JAN 2020. (18/01890-3, 18/19539-0)

SILVA MORETTO, JESSICA APARECIDA; RUEDA FURLAN, JOAO PEDRO; BRAZ, VANIA SANTOS; BITENCOURT, TAMIRES APARECIDA; STEHLING, ELIANA GUEDES. Presence of bla(OXA-48)-carrying IncF plasmid in an Escherichia fergusonii strain isolated from a sugarcane soil. Pedosphere, v. 30, n. 2, p. 293-294, APR 2020. (18/01890-3, 18/19539-0)

RUEDA FURLAN, JOAO PEDRO; STEHLING, ELIANA GUEDES. Molecular characterization of an extensively drug-resistant Acinetobacter baumannii isolated from a corn culture soil. Pedosphere, v. 31, n. 6, p. 973-976, OCT 2021. (18/01890-3, 18/19539-0)

RUEDA FURLAN, JOAO PEDRO; LAGE GALLO, INARA FERNANDA; LEONELLI PIRES DE CAMPOS, ANNA CAROLINA; NAVARRO, ARMANDO; TAKAYAMA KOBAYASHI, RENATA KATSUKO; NAKAZATO, GERSON; STEHLING, ELIANA GUEDES. Characterization of non-O157 Shiga toxin-producing Escherichia coli (STEC) obtained from feces of sheep in Brazil. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, v. 35, n. 9, SEP 2019. (18/19539-0)

FURLAN, JOAO PEDRO RUEDA; DOS SANTOS, LUCAS DAVID RODRIGUES; RAMOS, MICAELA SANTANA; GALLO, INARA FERNANDA LAGE; STEHLING, ELIANA GUEDES. Fecal cultivable aerobic microbiota of dairy cows and calves acting as reservoir of clinically relevant antimicrobial resistance genes. Brazilian Journal of Microbiology, v. 51, n. 3, APR 2020. (18/19539-0)

BARDAJI, DANAE KALA RODRIGUEZ; MORETTO, JESSICA APARECIDA SILVA; FURLAN, JOAO PEDRO RUEDA; STEHLING, ELIANA GUEDES. A mini-review: current advances in polyethylene biodegradation. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, v. 36, n. 2, FEB 14 2020. (18/19539-0)

RUEDA FURLAN, JOAO PEDRO; LAGE GALLO, INARA FERNANDA; PIRES DE CAMPOS, ANNA CAROLINA LEONELLI; PASSAGLIA, JAQUELINE; FALCAO, JULIANA PFRIMER; NAVARRO, ARMANDO; NAKAZATO, GERSON; STEHLING, ELIANA GUEDES. Molecular characterization of multidrug-resistant Shiga toxin-producing Escherichia coli harboring antimicrobial resistance genes obtained from a farmhouse. PATHOGENS AND GLOBAL HEALTH, v. 113, n. 6, p. 7-pg., 2019-11-22. (18/19539-0)

RUEDA FURLAN, JOAO PEDRO; RODRIGUES DOS SANTOS, LUCAS DAVID. Presence of Colistin Resistancemcr-4Gene and Clinically Relevant Antimicrobial Resistance Genes in Sand Samples from a Public Beach. WATER AIR AND SOIL POLLUTION, v. 231, n. 7, JUN 17 2020. (18/01890-3, 18/19539-0)

LOPES, RALF; FURLAN, JOAO PEDRO RUEDA; DOS SANTOS, LUCAS DAVID RODRIGUES; GALLO, INARA FERNANDA LAGE; STEHLING, ELIANA GUEDES. Colistin-Resistant mcr-1-Positive Escherichia coli ST131-H22 Carrying bla(CTX-M-15) and qnrB19 in Agricultural Soil. FRONTIERS IN MICROBIOLOGY, v. 12, APR 9 2021. (18/01890-3, 18/19539-0)

FURLAN, JOAO PEDRO RUEDA; GONZALEZ, IRYS HANY LIMA; RAMOS, PATRICIA LOCOSQUE; STEHLING, ELIANA GUEDES. International high-risk clone of multidrug-resistant CTX-M-8-producing Escherichia coli C-ST410 infecting an elephant (Loxodonta africana) in a zoo. JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, v. 22, p. 3-pg., 2020-09-01. (18/19539-0)

RUEDA FURLAN, JOAO PEDRO; STEHLING, ELIANA GUEDES. Draft genome sequence of a multidrug-resistant Escherichia coli ST189 carrying several acquired antimicrobial resistance genes obtained from Brazilian soil. JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, v. 17, p. 2-pg., 2019-06-01. (18/19539-0)