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Identification and molecular characterization of pathogenic microorganisms in powdered infant products

Grant number: 16/14107-0
Support type:Regular Research Grants
Duration: July 01, 2017 - December 31, 2019
Field of knowledge:Agronomical Sciences - Food Science and Technology
Principal Investigator:Dirce Yorika Kabuki
Grantee:Dirce Yorika Kabuki
Home Institution: Faculdade de Engenharia de Alimentos (FEA). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Due to the impossibility of breastfeeding in many cases, the food market was favored and the development of products for newborns and child nutrition is growing. A wide variety of these baby foods are produced in the country, such as those for neonates and babies. However, powdered infant formula has been identified as vehicle of microbiological contamination that can cause infections and diseases in infants (children less than one year old), since they have more susceptibility to pathogens. The aim of this project is to verify the presence of pathogenic bacteria in powered infant formula and infant cereals. Isolation and identification of micro-organisms, such as Salmonella sp., Cronobacter spp., Staphylococcus aureus, Bacillus cereus, and Escherichia coli, will be performed. It will also be verified whether the marketed products are in accordance with the microbiological standards established by Brazilian law. In addition, the pathogenic potential of individual species will be analyzed by Polymerase Chain Reaction (PCR) technique and multiplex PCR to detect genes encoding toxins and others virulence factors. Cronobacter spp. isolates will be identified by PCR and genetic profile will be analyzed by Multilocus Sequencing Typing (MLST). (AU)