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Contamination of sanitary conveyor use in poultry processing slaughterhouse, by Clostridium perfringens, Salmonella spp. and Escherichia coli, before and after sanitation with water spray

Grant number: 12/16655-3
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): November 01, 2012
Effective date (End): February 29, 2016
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Products of Animal Origin Inspection
Principal Investigator:Ruben Pablo Schocken-Iturrino
Grantee:Mariana Froner Casagrande
Home Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil


Nowadays, the Brazilian poultry industry is the third in the world raking in production of chicken meat, stating in 2010 the amount of 12.23 million tones. The consumption of animal protein increases every year, in 2009, the consumption per capita was 38.47 kg/year and in 2010, this value increased 44.09 kg/year (ABEF, 2011).Due to the increased consumption of poultry meat, it should be noted concern about the transmission of harmful pathogens to human health. The proper hygiene during the processing, storage and handling of food is the best method to avoid transmission of pathogens (PINTO, 1996).The main important pathogens found in poultry slaughterhouse are Clostridium spp., Listeria monocytogenes, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus, and microorganism of Enterobacteriaceae family, like Salmonella Typhimurium, Escherichia coli, Yersinia enterocolitica, , etc (MAROUANI-GADRI et al., 2009). Base on this data, the aim of this study is evaluate the use of water spray under pressure as a operational form of microbial control, analyzing the number of pathogenic bacteria present in conveyor in poultry processing. The analysis will be performed through quantification of Enterobacteriaceae and Clostridium spp. and isolation of Salmonella spp., Clostridium perfringens and Escherichia coli. After the use of traditional methods will be made PCR and identification of strains by PFGE technique. (AU)