Identification of genes codifying ESBLs, quinolone resistance and virulence in E. coli isoltaed from chickens, chicken meat and patients with urinary tract infections

• Grant number: 14/17184-0
• Support Opportunities: Regular Research Grants
• Duration: February 01, 2015 - April 30, 2017
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Mara Corrêa Lelles Nogueira
• Grantee: Mara Corrêa Lelles Nogueira
• Host Institution: Faculdade de Medicina de São José do Rio Preto (FAMERP). Secretaria de Desenvolvimento Econômico (São Paulo - Estado). São José do Rio Preto , SP, Brazil

Abstract

The urinary tract infection (UTI) by uropathogenic Escherichia coli (UPEC) resistant to beta-lactams and quinolones are an important public health problem, because these are the main recommended antibiotics and treatment failure can result in evolution for severe renal failure, sepsis and death. The main reservoir is UPEC the gastrointestinal (GI) tract, and infection occurs after the migration of the bacteria to the perianal region and subsequently into the urethra. In recent years, ITU has been considered as zoonoses and food-borne diseases, based on evidence that meat, especially chicken meat, are a vehicle for the transmission of UPEC from livestock to humans. Brazil is the largest producer of poultry meat in the world, and so, for reasons of public health and business, it is important to know the profile of virulence and resistance in E. coli isolated from chickens and chicken meat produced in the country. In a previous study by our group, E. coli harboring the genes blaCTX-M-2 and qnrB-like that codify resistance to extended spectrum beta-lactamas and decreased susceptibility to quinolones were isolated from samples of chicken meat. In this project, we propose to conduct concurrent evaluation of E. coli resistant to third generation cephalosporins and quinolones isolated from chicken meat, broiler chickens and patients with UTI. The profile of antimicrobial susceptibility genes responsible for ESBL production and resistance to quinolones, and the diversity of virulence genes will be investigated and compared. These bacteria will also be compared in terms of phylogenetic group and genetic similarity by PFGE and multilocus sequence typing (MLST). The results of this study will contribute to knowledge about the spread of resistance genes in UPEC and the role of the production chain of poultry meat in the spread of antimicrobial-resistant UPEC, and in future may be used for the development of food safety programs for the Brazilian meat productive chain. (AU)

Publicações científicas

(7)

(Referências obtidas automaticamente do Web of Science e do SciELO, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores)

CASELLA, TIAGO; HAENNI, MARISA; MADELA, NAIADY KONNO; DE ANDRADE, LETICIA KELLEN; PRADELA, LETICIA KALIR; DE ANDRADE, LEONARDO NEVES; DA COSTA DARINI, ANA LUCIA; MADEC, JEAN-YVES; LELLES NOGUEIRA, MARA CORREA. Extended-spectrum cephalosporin-resistant Escherichia coli isolated from chickens and chicken meat in Brazil is associated with rare and complex resistance plasmids and pandemic ST lineages. Journal of Antimicrobial Chemotherapy, v. 73, n. 12, p. 3293-3297, DEC 2018. (14/17184-0)

CASELLA, TIAGO; ZEQUINI DE MORAIS, ANDRESSA BATISTA; DE PAULA BARCELOS, DIEGO DINIZ; TOLENTINO, FERNANDA MODESTO; CERDEIRA, LOUISE TEIXEIRA; CAMPAGNARI BUENO, MARIA FERNANDA; FRANCISCO, GABRIELA RODRIGUES; DE ANDRADE, LEONARDO NEVES; DA COSTA DARINI, ANA LUCIA; GARCIA, DOROTI DE OLIVEIRA; et al. Draft genome sequence of a KPC-2-producing Klebsiella pneumoniae ST340 carrying bla(CTX-M-15) and bla(CTX-M-59) genes: a rich genome of mobile genetic elements and genes encoding antibiotic resistance. JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, v. 13, p. 2-pg., 2018-06-01. (14/17184-0)

MARTINS, EVELIN RODRIGUES; CAMPAGNARI BUENO, MARIA FERNANDA; FRANCISCO, GABRIELA RODRIGUES; CASELLA, TIAGO; GARCIA, DOROTI DE OLIVEIRA; CERDEIRA, LOUISE TEIXEIRA; GERBER, ALEXANDRA LEHMKUHL; PAULA DE ALMEIDA, LUIZ GONZAGA; LINCOPAN, NILTON; RIBEIRO DE VASCONCELOS, ANA TEREZA; et al. Genome and plasmid context of two rmtG-carrying Enterobacter hormaechei isolated from urinary tract infections in Brazil. JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, v. 20, p. 36-40, MAR 2020. (14/17184-0, 15/21325-0)

TOLENTINO, FERNANDA MODESTO; CAMPAGNARI BUENO, MARIA FERNANDA; FRANSCISCO, GABRIELA RODRIGUES; DE PAULA BARCELOS, DIEGO DINIZ; LOBO, SUZANA MARGARETH; MOREIRA BATISTA TOMAZ, FRANCIELI MAIRA; DA SILVA, NATAL SANTOS; DE ANDRADE, LEONARDO NEVES; CASELLA, TIAGO; DA COSTA DARINI, ANA LUCIA; et al. Endemicity of the High-Risk Clone Klebsiella pneumoniae ST340 Coproducing QnrB, CTX-M-15, and KPC-2 in a Brazilian Hospital. MICROBIAL DRUG RESISTANCE, v. 25, n. 4, DEC 2018. (12/21709-5, 14/17184-0)

MARTINS, EVELIN RODRIGUES; ESTOFOLETE, CASSIA FERNANDA; ZEQUINI, ANDRESSA BATISTA; CERDEIRA, LOUISE; GARCIA, DOROTI DE OLIVEIRA; CAMPAGNARI BUENO, MARIA FERNANDA; FRANCISCO, GABRIELA RODRIGUES; DE ANDRADE, LEONARDO NEVES; DA COSTA DARINI, ANA LUCIA; TOLENTINO, FERNANDA MODESTO; et al. Transfer of KPC-2 carbapenemase from Klebsiella pneumoniae to Enterobacter cloacae in a patient receiving meropenem therapy. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, v. 88, n. 3, p. 287-289, JUL 2017. (14/17184-0)

MARTINS, EVELIN RODRIGUES; CASELLA, TIAGO; CAMPAGNARI BUENO, MARIA FERNANDA; FRANCISCO, GABRIELA RODRIGUES; TOLENTINO, FERNANDA MODESTO; TEGON DE FREITAS, ANA CAROLINA; CERDEIRA, LOUISE; COSTA, MONALISA NOGUEIRA; CEVADA, CARLA; LINCOPAN, NILTON; et al. Draft genome sequence of an aminoglycoside-resistant RmtD2-producing Enterobacter cloacae subsp cloacae ST395 in Brazil. JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, v. 10, p. 308-309, SEP 2017. (15/21325-0, 14/17184-0)

CASELLA, TIAGO; CERDEIRA, LOUISE T.; FERNANDES, MIRIAM R.; SOUZA, TIAGO A.; HAENNI, MARISA; MADEC, JEAN-YVES; LINCOPAN, NILTON; NOGUEIRA, MARA C. L.. Draft genome sequence of a CTX-M-15-producing Escherichia coli ST345 from commercial chicken meat in Brazil. JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, v. 9, p. 2-pg., 2017-06-01. (14/17184-0, 15/21325-0)