Genome and plasmid context of two rmtG-carrying Enterobacter hormaechei isolated from urinary tract infections in Brazil

Author(s):Show less -

Martins, Evelin Rodrigues ^[1] ; Campagnari Bueno, Maria Fernanda ^[2] ; Francisco, Gabriela Rodrigues ^[2] ; Casella, Tiago ^{[1, 3]} ; Garcia, Doroti de Oliveira ^[2] ; Cerdeira, Louise Teixeira ^[4] ; Gerber, Alexandra Lehmkuhl ^[5] ; Paula de Almeida, Luiz Gonzaga ^[5] ; Lincopan, Nilton ^[4] ; Ribeiro de Vasconcelos, Ana Tereza ^[5] ; Nogueira, Mara Correa Lelles ^[1] ; Estofolete, Cassia Fernanda ^[3]

Affiliation

^[1] Fac Med Sao Jose do Rio Preto, Ctr Invest Microrganismos, Av Brigadeiro Faria Lima 5416, BR-15090000 Sao Jose Do Rio Preto, SP - Brazil

^[2] Adolfo Lutz Inst, Ctr Bacteriol, Sao Paulo - Brazil

^[3] Hosp Base, Sao Jose Do Rio Preto, SP - Brazil

^[4] Univ Sao Paulo, Dept Microbiol, Sao Paulo - Brazil

^[5] Lab Nacl Comp Cient, Petropolis, RJ - Brazil

• Document type: Journal article
• Source: JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE; v. 20, p. 36-40, MAR 2020.
• Web of Science Citations: 0

Abstract

Objectives: Enterobacter hormaechei is an important causative agent of severe infections in critically ill patients. Aminoglycosides are among the main antibiotics for the treatment of E. hormaechei infections, however the development of antimicrobial resistance is an increasing problem. RmtG is a 16S rRNA methyltransferase, a class of enzymes conferring high-level resistance to clinically relevant aminoglycosides. The aim of this study was to characterise the full genetic context of plasmids harbouring the rmtG gene in two aminoglycoside-resistant E. hormaechei isolated in Brazil. Methods: ThermtG-harbouring plasmids were transferred to an Escherichia coli J53 recipient strain and were fully sequenced using a MiSeq sequencing system. Complete genome assemblies were accomplished using a combination of Newbler v.3.0, SPAdes 3.10.0 and phrap/cross\_match programs. Plasmid sequences were annotated using RAST server and were then manually curated using BLAST databases and ISfinder. Easyfig 2.0 was used to map and compare regions of interest containing rmtG in both plasmids. Results: Both isolates carried thermtG gene on an IncA/C plasmid of (similar to)152 kb and (similar to)235 kb, respectively, associated with a Tn3 transposon. The plasmids contain a transfer region as well as genes involved in plasmid stability and resistance to beta-lactams, sulfonamides and quaternary ammonium compounds. One of the plasmids also carried the mrk operon encoding type 3 fimbriae. Conclusion: This first detection ofrmtG in E. hormaechei supports the ability for horizontal transfer. The location in complex genetic platforms carried by Tn3 transposons in IncA/C plasmids may facilitate dissemination to other Gram-negative pathogens, further limiting treatment options. (C) 2019 Published by Elsevier Ltd on behalf of International Society for Antimicrobial Chemotherapy. (AU)

• FAPESP's process: 14/17184-0 - Identification of genes codifying ESBLs, quinolone resistance and virulence in E. coli isoltaed from chickens, chicken meat and patients with urinary tract infections
• Grantee: Mara Corrêa Lelles Nogueira
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/21325-0 - Comparative analysis of the resistome of Klebsiella pneumoniae XDR (NDM-1/KPC-2) belonging to the endemic high-risk clonal complex CC258
• Grantee: Louise Teixeira Cerdeira
• Support Opportunities: Scholarships in Brazil - Doctorate