Advanced search
Start date
Betweenand

Comparative analysis of the resistome of Klebsiella pneumoniae XDR (NDM-1/KPC-2) belonging to the endemic high-risk clonal complex CC258

Grant number: 15/21325-0
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): March 01, 2016
Effective date (End): November 03, 2018
Field of knowledge:Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
Principal Investigator:Nilton Erbet Lincopan Huenuman
Grantee:Louise Teixeira Cerdeira
Home Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated scholarship(s):17/25909-2 - Comparative genomic analysis of Klebsiella pneumoniae belonging to the endemic high-risk clonal complex CC258 (ST11, ST437, ST340, ST258) in the Human-Animal-Environment interface in Brazil, BE.EP.DR

Abstract

The dissemination of Klebsiella pneumoniae strains showing a multidrug-resistant (MDR) or extreme drug resistance (XDR) profile is a worldwide issue that for countries with a high prevalence of infection it has acquired an endemic character. In Brazil, previous studies have shown that endemic genotypes, within many hospitals in the country, are associated with the production of KPC-2-type carbapenemases, with a rapid adaptation of high-risk clonal complexes (CCs) (eg., CC258). Additionally, to make matters worse for public health, recent studies have reported the presence of these clonal complexes in urban aquatic environments and food-producing animals. Most likely the genetic versatility of CC258 has contributed in this process of adaptation and persistence in the different ecosystems, so far studied, indeed, strains belonging to CC258 are largely considered responsible for the global spread of KPC. This study aims to conduct a pan-resistoma comparative analysis of K. pneumoniae XDR strains (NDM-1/KPC-2), belonging to the high-risk clonal complex CC258, endemic in Brazil. Strains of K. pneumoniae KPC-2/ST340, previously isolated from clinical cases, urban aquatic environments and farm animals will be subjected to complete genome sequencing with subsequent analysis of the pan-resistoma using complete and draft genome sequences, previuosly published, and different strategies and bioinformatics tools for assembly and analysis. The results may contribute to creating a platform that can be used by several researchers, for purposes of diagnosis, therapeutic and epidemiological control, among others; elucidating "a priori" genetic aspects of adaptation, antimicrobial resistance and virulence of this endemic clone.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MARTINS, EVELIN RODRIGUES; CAMPAGNARI BUENO, MARIA FERNANDA; FRANCISCO, GABRIELA RODRIGUES; CASELLA, TIAGO; GARCIA, DOROTI DE OLIVEIRA; CERDEIRA, LOUISE TEIXEIRA; GERBER, ALEXANDRA LEHMKUHL; PAULA DE ALMEIDA, LUIZ GONZAGA; LINCOPAN, NILTON; RIBEIRO DE VASCONCELOS, ANA TEREZA; NOGUEIRA, MARA CORREA LELLES; ESTOFOLETE, CASSIA FERNANDA. Genome and plasmid context of two rmtG-carrying Enterobacter hormaechei isolated from urinary tract infections in Brazil. JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, v. 20, p. 36-40, MAR 2020. Web of Science Citations: 0.
FUGA, BRUNA; FERREIRA, MELINA LORRAINE; CERDEIRA, LOUISE TEIXEIRA; DE CAMPOS, PAOLA AMARAL; DIAS, VINICIUS LOPES; ROSSI, IARA; MACHADO, LUIZ GUSTAVO; LINCOPAN, NILTON; GONTIJO-FILHO, PAULO PINTO; RIBAS, ROSINEIDE MARQUES. Novel small IncX3 plasmid carrying the bla(KPC-2) gene in high-risk Klebsiella pneumoniae ST11/CG258. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, v. 96, n. 2 FEB 2020. Web of Science Citations: 0.
MARTINS, EVELIN RODRIGUES; CASELLA, TIAGO; CAMPAGNARI BUENO, MARIA FERNANDA; FRANCISCO, GABRIELA RODRIGUES; TOLENTINO, FERNANDA MODESTO; TEGON DE FREITAS, ANA CAROLINA; CERDEIRA, LOUISE; COSTA, MONALISA NOGUEIRA; CEVADA, CARLA; LINCOPAN, NILTON; GARCIA, DOROTI DE OLIVEIRA; LELLES NOGUEIRA, MARA CORREA. Draft genome sequence of an aminoglycoside-resistant RmtD2-producing Enterobacter cloacae subsp cloacae ST395 in Brazil. JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, v. 10, p. 308-309, SEP 2017. Web of Science Citations: 1.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.