The dissemination of Klebsiella pneumoniae strains showing a multidrug-resistant (MDR) or extreme drug resistance (XDR) profile is a worldwide issue that for countries with a high prevalence of infection it has acquired an endemic character. In Brazil, previous studies have shown that endemic genotypes, within many hospitals in the country, are associated with the production of KPC-2-type carbapenemases, with a rapid adaptation of high-risk clonal complexes (CCs) (eg., CC258). Additionally, to make matters worse for public health, recent studies have reported the presence of these clonal complexes in urban aquatic environments and food-producing animals. Most likely the genetic versatility of CC258 has contributed in this process of adaptation and persistence in the different ecosystems, so far studied, indeed, strains belonging to CC258 are largely considered responsible for the global spread of KPC. This study aims to conduct a pan-resistoma comparative analysis of K. pneumoniae XDR strains (NDM-1/KPC-2), belonging to the high-risk clonal complex CC258, endemic in Brazil. Strains of K. pneumoniae KPC-2/ST340, previously isolated from clinical cases, urban aquatic environments and farm animals will be subjected to complete genome sequencing with subsequent analysis of the pan-resistoma using complete and draft genome sequences, previuosly published, and different strategies and bioinformatics tools for assembly and analysis. The results may contribute to creating a platform that can be used by several researchers, for purposes of diagnosis, therapeutic and epidemiological control, among others; elucidating "a priori" genetic aspects of adaptation, antimicrobial resistance and virulence of this endemic clone.
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