Characterization of Plasmids Associated with the Spread of the Colistin Resistance mcr-1 gene in Clinically Significant Enterobacteriaceae from South American and European Countries

• Grant number: 17/16754-5
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: January 10, 2018
• End date: April 24, 2018
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Nilton Erbet Lincopan Huenuman
• Grantee: Miriam Rodriguez Fernandes
• Supervisor: Alessandra Carattoli
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Institution abroad: Istituto Superiore di Sanità (ISS), Italy
• Associated to the scholarship: 15/13527-2 - Ancestral relationship, pan-resistoma plasmid of Escherichia coli producing b-lactamases of extended spectrum (CTX-M) and of plasmid curing methods, BP.DR

Abstract

The emergence and rapid dissemination of the plasmid-mediated mcr-1 gene among colistin-resistant Enterobacteriaceae has created an urgent need to elucidate the mechanisms involved in mcr-1 gene transfer. The increased incidences of the mcr-1 gene and its reports at global scale have revealed the hazard of the plasmid-mediated colistin-resistant in 44 countries, so far. Furthermore, plasmid carrying mcr-1 gene has been identified from different sources, including humans, livestock, companion animals, wild-animals, food, and aquatic environments, which reinforces the ubiquity of these plasmids. To our knowledge, mcr-1-harbouring plasmids have been grouped into eight replicon types: IncI2, IncX4, IncHI1, IncHI2, IncF, IncFI, IncFII, and IncP. Interestingly, IncX4-type plasmids carrying the mcr-1 gene have disseminated in a pandemic scale, being reported in 15 countries. In Brazil, IncX4 plasmids carrying mcr-1 have been identified among ESBL (CTX-M)- and KPC-2-producing Escherichia coli and Klebsiella pneumoniae from healthy livestock, infected human, sea birds, chicken meat and marine environment, in ten different states; whereas the presence of others worldwide disseminated plasmids has not been reported in Brazil. The present study aims to characterize and compare plasmids carrying mcr-1 gene occurring in Enterobacteriaceae recovered from clinical and non-clinical samples in South American and European countries.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ESPINAL, P.; NUCLEO, E.; CALTAGIRONE, M.; MARCHETTI, V. MATTIONI; FERNANDES, M. R.; BISCARO, V; RIGOLI, R.; CARATTOLI, A.; MIGLIAVACCA, R.; VILLA, L.. Genomics of Klebsiella pneumoniae ST16 producing NDM-1, CTX-M-15, and OXA-232. Clinical Microbiology and Infection, v. 25, n. 3, MAR 2019. (17/16754-5)