BACTERIAL PHOTOINACTIVATION OF MASTITIS PATHOGENS BY BLUE LIGHT: MECHANISMS OF ACTION AND CELL SECURITY - PRE-CLINICAL STUDY

• Grant number: 16/25095-2
• Support Opportunities: Scholarships in Brazil - Doctorate
• Effective date (Start): April 01, 2017
• Effective date (End): September 30, 2019
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Fabio Celidonio Pogliani
• Grantee: Carolina dos Anjos
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated scholarship(s): 19/10851-4 - SYNERGISTIC ACTIVITY OF BLUE LIGHT IN COMBINATION WITH CHEMOTHERAPEUTIC ANTIMICROBIAL AGAINST DRUG-RESISTANT PATHOGENS, BE.EP.DR

Abstract

Among the diseases that most compromise dairy production, bovine mastitis plays a pivotal role. Characterized as an inflammatory process of the mammary gland, mainly of bacterial origin, it is considered the main disease that affects dairy cattle, causing damages due to expenses with treatments and specialized labor, milk discharge, functional loss of mammary glands, and death of the animal as well. As an alternative to the use of antibiotics in the treatment and control of bacterial pathogens of bovine mastitis, blue light photoinactivation has proved to be a therapeutic option, with no selection of resistant microorganisms, absence of residues and milk discharges as well as environmental contamination. There are no studies that clearly describe the mechanisms of action of this therapy and the safety to bovine mammary epithelium, but it is believed that blue light induces excitation of endogenous bacterial porphyrins, leading to the generation of free radicals and singlet oxygen, acting simultaneously in several biomolecules of high importance to cellular functions and inducing bacterial death (photoinactivation). Thus, it becomes paramount to elucidate the biological mechanisms of the photoinactivation by the blue light, to evaluate the selectivity of this therapy to the bacterias, and identify the safe dosimetry levels that allow the viability of mammary epithelial cells.

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DOS ANJOS, CAROLINA; SABINO, CAETANO P.; SELLERA, FABIO P.; ESPOSITO, FERNANDA; POGLIANI, FABIO C.; LINCOPAN, NILTON. Hypervirulent and hypermucoviscous strains of Klebsiella pneumoniae challenged by antimicrobial strategies using visible light. INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, v. 56, n. 1, JUL 2020. (19/10851-4, 16/25095-2, 19/15578-4, 17/22406-0)

DOS ANJOS, CAROLINA; SELLERA, FABIO PARRA; GARGANO, RONALDO GOMES; LINCOPAN, NIKON; POGLIANI, FABIO CELIDONIO; RIBEIRO, MARCIO GARCIA; JAGIELSKI, TOMASZ; SABINO, CAETANO PADIAL. Algicidal effect of blue light on pathogenic Prototheca species. Photodiagnosis and Photodynamic Therapy, v. 26, p. 210-213, JUN 2019. (16/25095-2)

DOS ANJOS, CAROLINA; LEANSE, LEON G.; RIBEIRO, MARTHA S.; SELLERA, FABIO P.; DROPA, MILENA; ARANA-CHAVEZ, VICTOR E.; LINCOPAN, NILTON; BAPTISTA, MAURICIO S.; POGLIANI, FABIO C.; DAI, TIANHONG; et al. New Insights into the Bacterial Targets of Antimicrobial Blue Light. MICROBIOLOGY SPECTRUM, v. 11, n. 2, p. 13-pg., 2023-02-21. (16/25095-2, 19/10851-4)

DOS ANJOS, CAROLINA; SELLERA, FABIO P.; RIBEIRO, MARTHA S.; BAPTISTA, MAURICIO S.; POGLIANI, FABIO C.; LINCOPAN, NILTON; SABINO, CAETANO P.. Antimicrobial blue light and photodynamic therapy inhibit clinically relevant 8-lactamases with extended-spectrum (ESBL) and carbapenemase activity. Photodiagnosis and Photodynamic Therapy, v. 32, DEC 2020. (16/25095-2, 19/10851-4, 17/22406-0)

DOS ANJOS, CAROLINA; SABINO, CAETANO P.; BUERIS, VANESSA; FERNANDES, MIRIAM R.; POGLIANI, FABIO C.; LINCOPAN, NILTON; SELLERA, FABIO P.. Antimicrobial blue light inactivation of international clones of multidrug-resistant Escherichia coli ST10, ST131 and ST648. Photodiagnosis and Photodynamic Therapy, v. 27, p. 51-53, SEP 2019. (15/13527-2, 16/08593-9, 16/25095-2)

SABINO, CAETANO PADIAL; WAINWRIGHT, MARK; RIBEIRO, MARTHA SIMOES; SELLERA, FABIO PARRA; DOS ANJOS, CAROLINA; BAPTISTA, MAURICIO DA SILVA; LINCOPAN, NILTON. Global priority multidrug-resistant pathogens do not resist photodynamic therapy. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 208, JUL 2020. (16/08593-9, 17/22406-0, 13/07937-8, 16/25095-2)

SABINO, CAETANO P.; RIBEIRO, MARTHA S.; WAINWRIGHT, MARK; DOS ANJOS, CAROLINA; SELLERA, FABIO P.; DROPA, MILENA; NUNES, NATHALIA B.; BRANCINI, GUILHERME T. P.; BRAGA, GILBERTO U. L.; ARANA-CHAVEZ, VICTOR E.; et al. The Biochemical Mechanisms of Antimicrobial Photodynamic Therapy(1). Photochemistry and Photobiology, v. N/A, p. 9-pg., 2022-08-12. (16/25095-2, 17/22406-0, 16/08593-9, 13/07937-8, 19/10851-4)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

ANJOS, Carolina dos. Antimicrobial blue light: bacterial targets and mechanism of action. 2021. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) São Paulo.