The effectiveness of curcumin-mediated antimicrobial photodynamic therapy depends on pre-irradiation and biofilm growth times

Autor(es)

Cusicanqui Mendez, Daniela Alejandra ^[1] ; Gutierrez, Eliezer ^[1] ; Chaves Lamarque, Giuliana Campos ^[1] ; Rizzato, Veridiana Lopes ^[1] ; Rabelo Buzalaf, Marilia Afonso ^[2] ; Andrade Moreira Machado, Maria Aparecida ^[1] ; Cruvinel, Thiago ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Pediat Dent Orthodont & Publ Hlth, Bauru Sch Dent, Alameda Dr Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP - Brazil

^[2] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Bauru, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Photodiagnosis and Photodynamic Therapy; v. 27, p. 474-480, SEP 2019.
• Citações Web of Science: 0

Resumo

Background: The aim of this study was to determine the influence of distinct pre-irradiation times (PIT) of curcumin on the effectiveness of antimicrobial photodynamic therapy (aPDT) against intact dentin caries biofilms grown for 3 or 5 days. Methods: The microcosm biofilms grew on non-fluorescent glass blocks immersed in McBain medium with 1% sucrose, using microaerophilic conditions at 37 degrees C for 3 or 5 days. The biofilms were treated by the association of 600 mu mol.L-1 curcumin using different pre-irradiation times (1, 2 or 5 min) combined with 0 or 75 J.cm(-2) blue LED. Then, the vitality of biofilms was determined by confocal scanning laser microscopy (CSLM), after being stained with the mixture of ethidium bromide and fluorescein diacetate. Statistical analysis was performed by two-way ANOVA and post-hoc Tukey tests, after arcsine transformation (P < 0,05). Results: In comparison to control, curcumin alone (PIT = 5 min) and all combinations of curcumin and LED reduced significantly the vitality of 3-day biofilms. Distinctly, only curcumin plus LED using PITs of 2 or 5 min were effective in reducing the vitality of 5-day biofilms. Conclusion: Curcumin-mediated aPDT significantly decreased the vitality of intact dentin caries microcosms grown during 3 or 5 days, although successful treatments of 5-day biofilms required longer PITs in comparison to their counterparts. (AU)

• Processo FAPESP: 14/22238-1 - Efeito da terapia fotodinâmica antimicrobiana sobre microcosmos de lesões de cárie em dentina
• Beneficiário: Eliézer Gutierres
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 15/08595-9 - Efeito da terapia fotodinâmica antimicrobiana sobre microrganismos específicos provenientes de microcosmos de lesões de cárie em dentina
• Beneficiário: Daniela Alejandra Cusicanqui Méndez
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 14/10897-0 - Efeito da terapia fotodinâmica antimicrobiana sobre microcosmos de lesões de cárie em dentina
• Beneficiário: Thiago Cruvinel da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular