The effectiveness of curcumin-mediated antimicrobial photodynamic therapy depends on pre-irradiation and biofilm growth times

Author(s)

Cusicanqui Mendez, Daniela Alejandra ^[1] ; Gutierrez, Eliezer ^[1] ; Chaves Lamarque, Giuliana Campos ^[1] ; Rizzato, Veridiana Lopes ^[1] ; Rabelo Buzalaf, Marilia Afonso ^[2] ; Andrade Moreira Machado, Maria Aparecida ^[1] ; Cruvinel, Thiago ^[1]

Affiliation

^[1] Univ Sao Paulo, Dept Pediat Dent Orthodont & Publ Hlth, Bauru Sch Dent, Alameda Dr Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP - Brazil

^[2] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Bauru, SP - Brazil

• Document type: Journal article
• Source: Photodiagnosis and Photodynamic Therapy; v. 27, p. 474-480, SEP 2019.
• Web of Science Citations: 0

Abstract

Background: The aim of this study was to determine the influence of distinct pre-irradiation times (PIT) of curcumin on the effectiveness of antimicrobial photodynamic therapy (aPDT) against intact dentin caries biofilms grown for 3 or 5 days. Methods: The microcosm biofilms grew on non-fluorescent glass blocks immersed in McBain medium with 1% sucrose, using microaerophilic conditions at 37 degrees C for 3 or 5 days. The biofilms were treated by the association of 600 mu mol.L-1 curcumin using different pre-irradiation times (1, 2 or 5 min) combined with 0 or 75 J.cm(-2) blue LED. Then, the vitality of biofilms was determined by confocal scanning laser microscopy (CSLM), after being stained with the mixture of ethidium bromide and fluorescein diacetate. Statistical analysis was performed by two-way ANOVA and post-hoc Tukey tests, after arcsine transformation (P < 0,05). Results: In comparison to control, curcumin alone (PIT = 5 min) and all combinations of curcumin and LED reduced significantly the vitality of 3-day biofilms. Distinctly, only curcumin plus LED using PITs of 2 or 5 min were effective in reducing the vitality of 5-day biofilms. Conclusion: Curcumin-mediated aPDT significantly decreased the vitality of intact dentin caries microcosms grown during 3 or 5 days, although successful treatments of 5-day biofilms required longer PITs in comparison to their counterparts. (AU)

• FAPESP's process: 14/22238-1 - Effect of antimicrobial photodynamic therapy on dentin caries microcosms
• Grantee: Eliézer Gutierres
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 15/08595-9 - Effect of antimicrobial photodynamic therapy on specific microorganisms from dentin caries microcosms
• Grantee: Daniela Alejandra Cusicanqui Méndez
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 14/10897-0 - Effect of antimicrobial photodynamic therapy on dentin caries microcosms
• Grantee: Thiago Cruvinel da Silva
• Support Opportunities: Regular Research Grants