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Effect of antimicrobial photodynamic therapy on specific microorganisms from dentin caries microcosms

Grant number: 15/08595-9
Support type:Scholarships in Brazil - Master
Effective date (Start): July 01, 2015
Effective date (End): February 28, 2017
Field of knowledge:Health Sciences - Dentistry - Pediatric Dentistry
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Thiago Cruvinel da Silva
Grantee:Daniela Alejandra Cusicanqui Méndez
Home Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil


This study will be performed in two steps. The first step aims to evaluate the effect of Antimicrobial Photodynamic Therapy (aPDT) on specific microbial groups from dentin caries microcosms. Dentin samples will be collected from cavitated lesions of ten 7-11 year-old children to grow biofilms on bovine dentin specimens for 72 h. Subsequently, biofilms will be treated by association between curcumin and a blue LED (420 nm) or methylene blue and a red LED (630 nm). The viability of specific microbial groups will be determined by counts of Colony Forming Units of total microorganisms (Tryptic Soy Blood Agar), total streptococci (Mitis Salivarius Agar), mutans streptococci (Mitis Salivarius Agar plus bacitracin 0.2%), and lactobacilli (Rogosa Agar plus glacial acetic acid 0.13%). The method of enzymatic spectrophotometry will be performed to evaluate the concentration of L-lactate in the culture media. The quantification of total microorganisms, total streptococci, total lactobacilli, Prevotella spp., Bifidobacterium spp., Streptococcus mutans, Streptococcus intermedius, Lactobacillus acidophilus, Veillonella spp., Propionibacterium spp. e Actinomyces naeslundii will be performed by Real Time-PCR. The second step will evaluate the effect of distinct protocols of aPDT on the viability of microorganisms from different biofilm depths. Intact biofilms, treated or not with aPDT, will be stained with a mixture of ethidium bromide and fluorescein diacetate and assessed by confocal laser scanning microscopy. Statistical analysis will be based on the normality and homogeneity of the data. P values <0.05 will be considered significant. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CUSICANQUI MENDEZ, DANIELA ALEJANDRA; GUTIERREZ, ELIEZER; CHAVES LAMARQUE, GIULIANA CAMPOS; RIZZATO, VERIDIANA LOPES; RABELO BUZALAF, MARILIA AFONSO; ANDRADE MOREIRA MACHADO, MARIA APARECIDA; CRUVINEL, THIAGO. The effectiveness of curcumin-mediated antimicrobial photodynamic therapy depends on pre-irradiation and biofilm growth times. Photodiagnosis and Photodynamic Therapy, v. 27, p. 474-480, SEP 2019. Web of Science Citations: 0.
CUSICANQUI MENDEZ, DANIELA ALEJANDRA; GUTIERRES, ELIEZER; DIONISIO, EVANDRO JOSE; RABELO BUZALAF, MARILIA AFONSO; OLIVEIRA, RODRIGO CARDOSO; ANDRADE MOREIRA MACHADO, MARIA APARECIDA; CRUVINEL, THIAGO. Curcumin-mediated antimicrobial photodynamic therapy reduces the viability and vitality of infected dentin caries microcosms. Photodiagnosis and Photodynamic Therapy, v. 24, p. 102-108, DEC 2018. Web of Science Citations: 6.
CUSICANQUI MENDEZ, DANIELA ALEJANDRA; GUTIERREZ, ELIEZER; DIONISIO, EVANDRO JOSE; OLIVEIRA, THAIS MARCHINI; RABELO BUZALAF, MARILIA AFONSO; RIOS, DANIELA; APARECIDA ANDRADE MOREIRA MACHADO, MARIA; CRUVINEL, THIAGO. Effect of methylene blue-mediated antimicrobial photodynamic therapy on dentin caries microcosms. Lasers in Medical Science, v. 33, n. 3, p. 479-487, APR 2018. Web of Science Citations: 7.

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