Effect of antimicrobial photodynamic therapy on dentin caries microcosms

• Grant number: 14/22238-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2015
• End date: November 30, 2016
• Field of knowledge: Health Sciences - Dentistry - Pediatric Dentistry
• Principal Investigator: Thiago Cruvinel da Silva
• Grantee: Eliézer Gutierres
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

This study aims to evaluate the effect of Antimicrobial Photodynamic Therapy (aPDT) on specific microbial groups from dentin caries microcosms. Dentin samples will be collected from cavitated lesions of ten 7-11 year-old children to grow biofilms on bovine dentin specimens for 72 h. Subsequently, biofilms will be treated by association between curcumin and a blue LED (420 nm) or methylene blue and a red LED (630 nm). The viability of specific microbial groups will be determined by counts of Colony Forming Units of total microorganisms (Tryptic Soy Blood Agar), total streptococci (Mitis Salivarius Agar), mutans streptococci (Mitis Salivarius Agar plus bacitracin 0.2%), and lactobacilli (Rogosa Agar plus glacial acetic acid 0.13%). Statistical analysis will be based on the normality and homogeneity of the data. P values <0.05 will be considered significant.

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CUSICANQUI MENDEZ, DANIELA ALEJANDRA; CARDENAS CUELLAR, MARICEL ROSARIO; PEDRINHA, VICTOR FELIZ; VELASQUEZ ESPEDILLA, EVELYN GIULIANA; DE ANDRADE, FLAVIANA BOMBARDA; RODRIGUES, PATRICIA DE ALMEIDA; CRUVINEL, THIAGO. Effects of curcumin-mediated antimicrobial photodynamic therapy associated to different chelators against Enterococcus faecalis biofilms. Photodiagnosis and Photodynamic Therapy, v. 35, SEP 2021. (14/10897-0, 14/22238-1, 15/08595-9)

CUSICANQUI MENDEZ, DANIELA ALEJANDRA; GUTIERREZ, ELIEZER; CHAVES LAMARQUE, GIULIANA CAMPOS; RIZZATO, VERIDIANA LOPES; RABELO BUZALAF, MARILIA AFONSO; ANDRADE MOREIRA MACHADO, MARIA APARECIDA; CRUVINEL, THIAGO. The effectiveness of curcumin-mediated antimicrobial photodynamic therapy depends on pre-irradiation and biofilm growth times. Photodiagnosis and Photodynamic Therapy, v. 27, p. 474-480, SEP 2019. (14/22238-1, 15/08595-9, 14/10897-0)

CUSICANQUI MENDEZ, DANIELA ALEJANDRA; RIZZATO, VERIDIANA LOPES; CHAVES LAMARQUE, GIULIANA CAMPOS; DIONISIO, EVANDRO JOSE; RABELO BUZALAF, MARILIA AFONSO; RIOS, DANIELA; ANDRADE MOREIRA MACHADO, MARIA APARECIDA; CRUVINEL, THIAGO. Could a chelant improve the effect of curcumin-mediated photodynamic antimicrobial chemotherapy against dental intact biofilms?. Lasers in Medical Science, v. 34, n. 6, p. 1185-1192, AUG 2019. (14/22238-1)

CUSICANQUI MENDEZ, DANIELA ALEJANDRA; GUTIERREZ, ELIEZER; DIONISIO, EVANDRO JOSE; OLIVEIRA, THAIS MARCHINI; RABELO BUZALAF, MARILIA AFONSO; RIOS, DANIELA; APARECIDA ANDRADE MOREIRA MACHADO, MARIA; CRUVINEL, THIAGO. Effect of methylene blue-mediated antimicrobial photodynamic therapy on dentin caries microcosms. Lasers in Medical Science, v. 33, n. 3, p. 479-487, APR 2018. (15/08595-9, 14/10897-0, 14/22238-1)

CUSICANQUI MENDEZ, DANIELA ALEJANDRA; GUTIERRES, ELIEZER; DIONISIO, EVANDRO JOSE; RABELO BUZALAF, MARILIA AFONSO; OLIVEIRA, RODRIGO CARDOSO; ANDRADE MOREIRA MACHADO, MARIA APARECIDA; CRUVINEL, THIAGO. Curcumin-mediated antimicrobial photodynamic therapy reduces the viability and vitality of infected dentin caries microcosms. Photodiagnosis and Photodynamic Therapy, v. 24, p. 102-108, DEC 2018. (15/08595-9, 14/10897-0, 14/22238-1)