Characterizing phenol-removing consortia under methanogenic and sulfate-reducing conditions: potential metabolic pathways

Autor(es)

Gouvea de Godoi, Leandro Augusto ^[1] ; Fuess, Lucas Tadeu ^[1] ; Delforno, Tiago Palladino ^[2] ; Foresti, Eugenio ^[1] ; Rissato Zamariolli Damianovic, Marcia Helena ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sao Carlos Sch Engn EESC, Biol Proc Lab LPB, Av Joao Dagnone 1100, BR-13563120 Sao Carlos, SP - Brazil

^[2] Campinas Univ UNICAMP, Res Ctr Chem Biol & Agr CPQBA, Microbial Resources Div, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ENVIRONMENTAL TECHNOLOGY; v. 40, n. 24, p. 3216-3226, OCT 28 2019.
• Citações Web of Science: 1

Resumo

Phenol removal was investigated in anaerobic fixed-structured bed reactors, namely R1 and R2, treating synthetic wastewater simulating the soluble fraction of vinasse under strictly methanogenic (R1) and simultaneous methanogenic/sulfidogenic conditions (R2). Next-generation sequencing (Illumina MiSeq System) was used to further characterize the microbial communities in both systems. Phenol was completely and stably removed in R1 after a short operating period (approximate to 55 days). Conversely, phenol removal in R2 required a longer period for biomass acclimation (approximate to 125 days) to reach levels equivalent to R1. Volatile fatty acids (VFA) accumulation in R2, mainly due to the inhibition of the acetoclastic methanogenesis by sulfide, may have limited phenol removal in the initial operating phases, as intermediate steps from phenol degradation are thermodynamically dependent on the removal of acetate, hydrogen and bicarbonate. Overall, the potential for anaerobically removing phenol from complex wastewaters was confirmed, even at low phenol/COD ratios. 16S rRNA gene sequencing analysis showed a high correlation of taxonomic profile between R1 and the inoculum, whereas a lower correlation was observed between R2 and the inoculum samples. Functional inference further indicated that Syntrophus and Bacillus genera in R1 and Clostridium genus in both reactors possibly played a key-role in phenol degradation. (AU)

• Processo FAPESP: 09/15984-0 - Produção de bioenergia no tratamento de águas residuárias e adequação ambiental dos efluentes e resíduos gerados
• Beneficiário: Marcelo Zaiat
• Modalidade de apoio: Auxílio à Pesquisa - Temático