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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Cell Therapy: Effect of Locally Injected Mesenchymal Stromal Cells Derived from Bone Marrow or Adipose Tissue on Bone Regeneration of Rat Calvarial Defects

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Autor(es):
Freitas, Gileade P. [1] ; Lopes, Helena B. [1] ; Souza, Alann T. P. [1] ; Oliveira, Paula G. F. P. [1] ; Almeida, Adriana L. G. [1] ; Souza, Lucas E. B. [2] ; Coelho, Paulo G. [3, 4] ; Beloti, Marcio M. [1] ; Rosa, Adalberto L. [1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sch Dent Ribeirao Preto, Bone Res Lab, Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Hemotherapy Ctr Ribeirao Preto, Sao Paulo, SP - Brazil
[3] NYU, Coll Dent, Dept Biomat, New York, NY - USA
[4] NYU, Sch Med, Hanjorg Wyss Dept Plast Surg, New York, NY - USA
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: SCIENTIFIC REPORTS; v. 9, SEP 17 2019.
Citações Web of Science: 0
Resumo

Treatment of large bone defects is a challenging clinical situation that may be benefited from cell therapies based on regenerative medicine. This study was conducted to evaluate the effect of local injection of bone marrow-derived mesenchymal stromal cells (BM-MSCs) or adipose tissue-derived MSCs (AT-MSCs) on the regeneration of rat ca lvaria I defects. BM-MSCs and AT-MSCs were characterized based on their expression of specific surface markers; cell viability was evaluated after injection with a 21-G needle. Defects measuring 5 mm that were created in rat calvaria were injected with BM-MSCs, AT-MSCs, or vehicle-phosphate-buffered saline (Control) 2 weeks post-defect creation. Cells were tracked by bioluminescence, and 4 weeks post-injection, the newly formed bone was evaluated by mu CT, histology, nanoindentation, and gene expression of bone markers. BM-MSCs and AT-MSCs exhibited the characteristics of MSCs and maintained their viability after passing through the 21-G needle. Injection of both BM-MSCs and AT-MSCs resulted in increased bone formation compared to that in Control and with similar mechanical properties as those of native bone. The expression of genes associated with bone formation was higher in the newly formed bone induced by BM-MSCs, whereas the expression of genes involved in bone resorption was higher in the AT-MSC group. Cell therapy based on local injection of BM-MSCs or AT-MSCs is effective in delivering cells that induced a significant improvement in bone healing. Despite differences observed in molecular cues between BM-MSCs and AT-MSCs, both cells had the ability to induce bone tissue formation at comparable amounts and properties. These results may drive new cell therapy approaches toward complete bone regeneration. (AU)

Processo FAPESP: 14/26864-4 - Efeito de injeções locais de células-tronco mesenquimais ou osteoblastos na regeneração do tecido ósseo
Beneficiário:Gileade Pereira Freitas
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 15/21439-6 - Células-tronco mesenquimais e osteoblastos: avaliação das assinaturas moleculares e do potencial para regenerar tecido ósseo
Beneficiário:Adalberto Luiz Rosa
Linha de fomento: Auxílio à Pesquisa - Regular