| Texto completo | |
| Autor(es): |
de Freitas, Laura Marise
[1, 2]
;
Lorenzon, Esteban Nicolas
[3]
;
Cilli, Eduardo Maffud
[4]
;
de Oliveira, Kleber Thiago
[5]
;
Fontana, Carla Raquel
[1]
;
Mang, Thomas S.
[2]
Número total de Autores: 6
|
| Afiliação do(s) autor(es): | [1] Paulo State Univ, Sch Pharmaceut Sci, UNESP, Araraquara, SP - Brazil
[2] Univ Buffalo, Dept Oral & Maxillofacial Surg, Sch Dent Med, Buffalo, NY 14260 - USA
[3] Univ Fed Goias, Biol Sci Inst, Biochem & Mol Biol Dept, Campus 2 Samambaia, Goiania, Go - Brazil
[4] Sao Paulo State Univ, Inst Chem, UNESP, Araraquara, SP - Brazil
[5] Fed Univ Sao Carlos UFSCar, Dept Chem, Bioorgan Chem Lab, Sao Carlos, SP - Brazil
Número total de Afiliações: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | BIOFOULING; v. 35, n. 7, p. 742-757, AUG 9 2019. |
| Citações Web of Science: | 0 |
| Resumo | |
The self-produced extracellular polymeric matrix of biofilms renders them difficult to eliminate once they are established. This makes the inhibition of biofilm formation key to successful treatment of biofilm infection. Antimicrobial photodynamic therapy (aPDT) and antimicrobial peptides offer a new approach as antibiofilm strategies. In this study sub-lethal doses of aPDT (with chlorin-e6 (Ce6-PDT) or methylene blue (MB-PDT)) and the peptides AU (aurein 1.2 monomer) or (AU)(2)K (aurein 1.2 C-terminal dimer) were combined to evaluate their ability to prevent biofilm development by Enterococcus faecalis. Biofilm formation was assessed by resazurin reduction, confocal microscopy, and infrared spectroscopy. All treatments successfully prevented biofilm development. The (AU)(2)K dimer had a stronger effect, both alone and combined with aPDT, while the monomer AU had significant activity when combined with Ce6-PDT. Additionally, it is shown that the peptides bind to the lipoteichoic acid of the E. faecalis cell wall, pointing to a possible key mechanism of biofilm inhibition. | |
| Processo FAPESP: | 18/23015-7 - Terapia fotodinâmica antimicrobiana via irradiação em modo contínuo e chaveado contra Enterococcus faecalis e Cutibacterium acnes |
| Beneficiário: | Carla Raquel Fontana |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 14/24581-5 - Avaliação do papel da terapia fotodinâmica combinada a peptídeos antimicrobianos frente à resistência bacteriana |
| Beneficiário: | Laura Marise de Freitas |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 16/18378-8 - Modificação e aprimoramento da terapia fotodinâmica antimicrobiana no tratamento de infecções orais e de cabeça e pescoço |
| Beneficiário: | Laura Marise de Freitas |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Doutorado |