Photobleaching Efficiency Parallels the Enhancement of Membrane Damage for Porphyrazine Photosensitizers

Autor(es)

Tasso, Thiago T. ^[1] ; Schlothauer, Jan C. ^[1] ; Junqueira, Helena C. ^[1] ; Matias, Tiago A. ^[2] ; Araki, Koiti ^[2] ; Liandra-Salvador, Erica ^[3] ; Antonio, Felipe C. T. ^[3] ; Homem-de-Mello, Paula ^[3] ; Baptista, Mauricio S. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Chem Inst, Dept Biochem, BR-05508000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Chem Inst, Dept Fundamental Chem, BR-05508000 Sao Paulo - Brazil

^[3] Fed Univ ABC, Ctr Nat Sci & Humanities, BR-09210580 Santo Andre - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of the American Chemical Society; v. 141, n. 39, p. 15547-15556, OCT 2 2019.
• Citações Web of Science: 2

Resumo

Photostability is considered a key asset for photosensitizers (PS) used in medical applications as well as for those used in energy conversion devices. In light-mediated medical treatments, which are based on PS-induced harm to diseased tissues, the photoinduced cycle of singlet oxygen generation has always been considered to correlate with PS efficiency. However, recent evidence points to the fundamental role of contact-dependent reactions, which usually cause PS photobleaching. Therefore, it seems reasonable to challenge the paradigm of photostability versus PS efficiency in medical applications. We have prepared a series of Mg(II) porphyrazines (MgPzs) having similar singlet oxygen quantum yields and side groups with different electron-withdrawing strengths that fine-tune their redox properties. A detailed investigation of the photobleaching mechanism of these porphyrazines revealed that it is independent of singlet oxygen, occurring mainly via photoinduced electron abstraction of surrounding electron rich molecules (solvents or lipids), as revealed by the formation of an air-stable radical anion intermediate. When incorporated into phospholipid membranes, photobleaching of MgPzs correlates with the degree of lipid unsaturation, indicating that it is caused by an electron abstraction from the lipid double bond. Interestingly, upon comparing the efficiency of membrane photodamage between two of these MgPzs (with the highest and the lowest photobleaching efficiencies), we found that the higher the rate of PS photobleaching the faster the leakage induced in the membranes. Our results therefore indicate that photobleaching is a necessary step toward inflicting irreversible biological damage. We propose that the design of more efficient PS for medical applications should contemplate contact-dependent reactions as well as strategies for PS regeneration. (AU)

• Processo FAPESP: 16/15916-9 - Estudos de fotoestabilidade e fotosensibilização de octaarilporfirazinas halogenadas para aplicação em terapia fotodinâmica
• Beneficiário: Thiago Teixeira Tasso
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 17/23416-9 - Fotossensibilizadores: estudos de propriedades fundamentais e aplicações biológicas
• Beneficiário: Paula Homem-de-Mello
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/07937-8 - Redoxoma
• Beneficiário: Ohara Augusto
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs