MiR-455-3p, miR-150 and miR-375 are aberrantly expressed in salivary gland adenoid cystic carcinoma and polymorphous adenocarcinoma

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Brown, Amy L. ^[1] ; Al-Samadi, Ahmed ^[2] ; Sperandio, Marcelo ^[1] ; Soares, Andresa Borges ^[1] ; Teixeira, Lucas Novaes ^[1] ; Martinez, Elizabeth F. ^[1] ; Dias Demasi, Ana Paula ^[1] ; Araujo, Vera Cavalcanti ^[1] ; Leivo, Ilmo ^{[3, 4]} ; Salo, Tuula ^{[5, 6, 2, 7]} ; Passador-Santos, Fabricio ^[1]

Afiliação do(s) autor(es)

^[1] Inst Pesquisas Sao Leopoldo Mandic, Fac Sao Leopoldo Mandic, Dept Oral Pathol, Campinas, SP - Brazil

^[2] Univ Helsinki, Fac Med, Clinicum, Dept Oral & Maxillofacial Dis, Helsinki - Finland

^[3] Univ Turku, Dept Pathol, Inst Biomed, Turku - Finland

^[4] Turku Univ Hosp, Turku - Finland

^[5] Oulu Univ Hosp, Med Res Ctr, Oulu - Finland

^[6] Helsinki Univ Hosp, Helsinki - Finland

^[7] Univ Oulu, Canc & Translat Med Res Unit, Oulu - Finland

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ORAL PATHOLOGY & MEDICINE; v. 48, n. 9, p. 840-845, OCT 2019.
• Citações Web of Science: 2

Resumo

Background Adenoid cystic carcinoma (AdCC) and polymorphous adenocarcinoma (PAC) are included among the most common salivary gland cancers. They share clinical and histological characteristics, making their diagnosis challenging in specific cases. MicroRNAs (miRNA) are short, non-coding RNA sequences of 19-25 nucleotides in length that are involved in post-transcriptional protein expression. They have been shown to play important roles in neoplastic and non-neoplastic processes and have been suggested as diagnostic and prognostic markers. Methods This study, using quantitative RT-PCR, investigated miR-150, miR-455-3p and miR-375 expression, in order to identify a possible molecular distinction between AdCC and PAC. Results miRNA-150 and miRNA-375 expression was significantly decreased in AdCC and PAC compared with salivary gland tissue controls, whilst miRNA-455-3p showed significantly increased expression in AdCC when compared to PAC, (P < 0.05). miR-150, miR-357 and miR-455-3p expression in AdCC, PAC and control was not associated with age, gender nor with anatomic site (major and minor salivary glands) (P > 0.05). Conclusion MiR-455-3p could be used as a complimentary tool in the diagnosis of challenging AdCC cases. (AU)

• Processo FAPESP: 12/10382-5 - Estudo integrado de expressão gênica e de microRNAs em carcinomas mucoepidermoides de glândulas salivares
• Beneficiário: Fabricio Passador-Santos
• Modalidade de apoio: Auxílio à Pesquisa - Regular