| Texto completo | |
| Autor(es): |
Van Acker, Thibaut
[1]
;
Van Malderen, Stijn J. M.
[1, 2]
;
Colina-Vegas, Legna
[3]
;
Ramachandran, Ranjith K.
[4]
;
Vanhaecke, Frank
[1]
Número total de Autores: 5
|
| Afiliação do(s) autor(es): | [1] Univ Ghent, Dept Chem, Atom & Mass Spectrometry A&MS Res Unit, Campus Sterre, Krijgslaan 281-512, B-9000 Ghent - Belgium
[2] Univ Ghent, Dept Chem, Xray Microspect & Imaging XMI Res Unit, Campus Sterre, Krijgslaan 281-512, B-9000 Ghent - Belgium
[3] Univ Fed Sao Carlos, Dept Chem, Km 235, Sao Carlos, SP - Brazil
[4] Univ Ghent, Dept Solid State Sci, COCOON, Campus Sterre, Krijgslaan 281-S1, B-9000 Ghent - Belgium
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Journal of Analytical Atomic Spectrometry; v. 34, n. 10, p. 1957-1964, OCT 1 2019. |
| Citações Web of Science: | 0 |
| Resumo | |
This paper describes the possibility of controlling and reducing the laser energy density of nanosecond 193 nm laser radiation in order to selectively ablate biological material from a glass substrate for LA-ICP-MS bioimaging applications. Atomic force microscopy and optical profilometry were used to study the shape of single-shot craters in dried gelatin droplets, ablated at low energy (<500 mJ cm(-2)). These craters were characterized by straight walls and a `flat bottom'. Based on the ablated volume per pulse and corresponding ablation depth, the ablation threshold of the dried gelatin material was estimated at 44 mJ cm(-2) by relying on the Beer model derived from the Beer-Lambert law. Three different glass substrates, i.e. a microscope slide and coverslip of soda-lime-silica (SLS) glass with a slightly different bulk elemental composition and a borosilicate glass coverslip, were ablated using laser energy densities ranging from 150-730 mJ cm(-2) and typical instrument settings applied during LA-ICP-MS bioimaging. The signal intensity of Si-29, a minor isotope of Si which is a major matrix component of the glass surface, was monitored as a measure of material removal and was used to estimate ablation thresholds for the three glass substrates at 262, 181 and 104 mJ cm(-2), respectively. As a proof-of-concept, kidney tissue mounted onto a SLS glass microscope slide substrate and MDA-MB-231 tumor cells seeded on a SLS glass coverslip were selectively ablated by controlling the laser energy density to ensure soft ablation of the glass substrate, but provide hard ablation of the biological material. (AU) | |
| Processo FAPESP: | 17/23254-9 - Determinação quantitativa e imagem celular de Cu, Ru e Pt em células individuais usando Ablação a laser acoplado ao ICP-MS |
| Beneficiário: | Legna Andreina Colina Vegas |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado |