Lower Susceptibility of Laser-irradiated Ti-15Mo Surface to Methicillin-resistant Staphylococcus aureus Cells Adhesion

Autor(es)

Semeghini Guastaldi, Fernando Pozzi ^[1] ; de Foggi, Camila Cristina ^[2] ; Leal Santana, Luis Carlos ^[1] ; Vaz, Luis Geraldo ^[2] ; Vergani, Carlos Eduardo ^[2] ; Guastaldi, Antonio Carlos ^[3]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista UNESP, Fac Odontol Araraquara, Dept Diagnost & Cirurgia, Araraquara, SP - Brazil

^[2] Univ Estadual Paulista Unesp, Fac Odontol Araraquara, Dept Mat Dentarios & Protese, Araraquara, SP - Brazil

^[3] Univ Estadual Paulista Unesp, Inst Quim Araraquara, Dept Fisicoquim, Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MATERIALS RESEARCH-IBERO-AMERICAN JOURNAL OF MATERIALS; v. 22, n. 4 2019.
• Citações Web of Science: 0

Resumo

Extensive data reported the influence of the physicochemical properties on the bacterial adhesion in biomaterials, of which surface roughness of titanium (Ti) can dictates methicillin-resistant Staphylococcus aureus (MRSA) adhesion to orthopedic implants. Herein, we investigated the influence of the Yb:YAG laser texturing of titanium-15molybdenum (TiMo-L) surface on the MRSA (ATCC \#33591) cells adhesion and viability. The physicochemical properties and antibacterial performance of TiMo-L were compared to samples of laser-irradiated pure titanium (Ti-L). Polished samples (Ti-P and TiMo-P) were used as controls. Laser textured surfaces presented a high degree of hydrophilicity, an irregular-shaped cavity and a typical microstructured pattern, compared to the polished substrates. The laser irradiation reduced the peaks of molybdenum (Mo) in the surface of Ti-15Mo alloy, which is explained, at least in part, by the difference between the melting point of Ti (1.668 degrees C) and Mo (2.623 degrees C). Laser texturing raised the MRSA cells viability and statistically increased the bacterial adhesion to pure Ti (P < 0.01; Wilcoxon-Signed rank test) and Ti-15Mo alloy (P < 0.001; Paired t test). The TiMo-L surface was significantly less susceptible to MRSA cell adhesion compared to Ti-L substrate (P < 0.001; Paired t test). (AU)

• Processo FAPESP: 14/16688-4 - Desenvolvimento e avaliação biológica de um novo fosfato de cálcio com e sem selante de fibrina: estudo in vivo em coelhos e estudo in vitro
• Beneficiário: Fernando Pozzi Semeghini Guastaldi
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado