Resistance Breeding of Common Bean Shapes the Physiology of the Rhizosphere Microbiome

Autor(es)

Mendes, Lucas William ^{[1, 2]} ; de Chaves, Miriam Goncalves ^[2] ; Fonseca, Mariley de Cassia ^[2] ; Mendes, Rodrigo ^[3] ; Raaijmakers, Jos M. ^{[1, 4]} ; Tsai, Siu Mui ^[2]

Afiliação do(s) autor(es)

^[1] Netherlands Inst Ecol NIOO KNAW, Dept Microbial Ecol, Wageningen - Netherlands

^[2] Univ Sao Paulo, Cell & Mol Biol Lab, Ctr Nucl Energy Agr CENA, Piracicaba - Brazil

^[3] Embrapa Meio Ambiente, Jaguariuna - Brazil

^[4] Leiden Univ, Inst Biol Leiden, Leiden - Netherlands

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN MICROBIOLOGY; v. 10, OCT 1 2019.
• Citações Web of Science: 0

Resumo

The taxonomically diverse rhizosphere microbiome contributes to plant nutrition, growth and health, including protection against soil-borne pathogens. We previously showed that breeding for Fusarium-resistance in common bean changed the rhizosphere microbiome composition and functioning. Here, we assessed the impact of Fusarium-resistance breeding in common bean on microbiome physiology. Combined with metatranscriptome data, community-level physiological profiling by Biolog EcoPlate analyses revealed that the rhizosphere microbiome of the Fusarium-resistant accession was distinctly different from that of the Fusarium-susceptible accession, with higher consumption of amino acids and amines, higher metabolism of xylanase and sialidase, and higher expression of genes associated with nitrogen, phosphorus and iron metabolism. The resistome analysis indicates higher expression of soxR, which is involved in protecting bacteria against oxidative stress induced by a pathogen invasion. These results further support our hypothesis that breeding for resistance has unintentionally shaped the assembly and activity of the rhizobacterial community toward a higher abundance of specific rhizosphere competent bacterial taxa that can provide complementary protection against fungal root infections. (AU)

• Processo FAPESP: 14/03217-3 - Microbioma da rizosfera de feijão resistente ao patógeno de solo Fusarium oxysporum
• Beneficiário: Lucas William Mendes
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 15/00251-9 - Análise do microbioma associado ao sistema radicular de feijão
• Beneficiário: Lucas William Mendes
• Linha de fomento: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado