A new perspective of lactatogenesis by isolated adipocytes

Autor(es)

Borghi, Filipy ^[1] ; Morais, Camila L. ^[1] ; Silva, Carolina ^[1] ; da Silva, Priscila C. ^[1] ; Ishizu, Larissa Y. ^[1] ; Costa, Gustavo T. ^[1] ; Grassi-Kassisse, Dora M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Biol, Dept Struct & Funct Biol, LABEEST Lab Stress Study, UNICAMP, Cidade Univ Zeferino Vaz, Rua Monteiro Lobato 255, BR-13083862 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Molecular and Cellular Endocrinology; v. 498, DEC 1 2019.
• Citações Web of Science: 0

Resumo

Increased adipose tissue mass exhibited greater capacity of glucose transformation in lactate, highlighting lactatogenesis as a crucial factor in body size. Classically, lactate produced by isolated adipocytes are expressed per million of cells and were never correlated with their size. Spontaneously hypertensive rats (SHR) have a lower body weight and smaller adipocytes when compared to Wistar-Kyoto. We evaluated basal lactate by epididymal 15-weeks-old isolated adipocytes of SHR, Wistar-Kyoto and Wistar. Basal lactate was similar when expressed by one million cells. However, SHR adipocytes were smaller, so we adjusted the results by cell volume and SHR showed higher basal lactate production which was directly endorsed by hyperlactatemia in the presented conditions. Thereby, we suggest a new perspective on lactatogenesis analysis by adipocytes, which could be linked to the receptors density and associate enzymes. Moreover, we showed that the thin and hypertensive rats can be hyperlactemic in fasting conditions. (AU)

• Processo FAPESP: 14/17538-6 - Análise da expressão de proteínas relacionadas ao metabolismo de adipócitos e reatividade vascular de ratos hipertensos e o envolvimento das adipocinas
• Beneficiário: Dora Maria Grassi-Kassisse
• Modalidade de apoio: Auxílio à Pesquisa - Regular