Biosensing of D-dimer, making the transition from the central hospital laboratory to bedside determination

Autor(es)

Tasic, Nikola ^[1] ; Paixao, Thiago R. L. C. ^[1] ; Goncalves, Luis Moreira ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Quim, Dept Quim Fundamental, Ave Prof Lineu Prestes 748, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Talanta; v. 207, JAN 15 2020.
• Citações Web of Science: 0

Resumo

Since the disclosure of the fibrinogen degradation mechanism, around half a century ago, a significant number of papers have been published related to the clinical relevance of D-dimer, a molecule immune to additional enzymatic decomposition by plasmin. Due to the obliquity of regulating blood coagulation in pathological events, the number of diseases and conditions associated with abnormal levels of D-dimer includes deep vein thrombosis, pulmonary embolism, sepsis, myocardial infarction, disseminated intravascular coagulation, among many others. D-dimer not only is an important player in medical diagnosis but also its role as a prognosis biomarker is being revealed. However, the number of analytical alternative methods has not accompanied this trend, even though novel simple point-of-care devices would certainly boost the relevance of D-dimer in emergency medicine. Some reasons for that could be related to the fact that D-dimer is a challenging analyte present in complex samples like blood. In this manuscript, subsequent to a fibrinogen degradation process introduction, it is provided a historical overview of the early D-dimer assays, followed by an extended focus on innovative solutions, with a spotlight on the electrochemical bioanalytical devices. The discussion is accompanied with a critical analysis and concluding thoughts concerning future perspectives. (AU)

• Processo FAPESP: 17/10522-5 - Desenvolvimento de métodos alternativos para fabricação de sensores eletroquímicos em papel
• Beneficiário: Thiago Regis Longo Cesar da Paixão
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/08782-1 - Sensores químicos miniaturizados e integrados: novas plataformas de fabricação para aplicações biológicas, clínicas e ambientais
• Beneficiário: Mauro Bertotti
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/13922-7 - Desenvolvimento de um biosensor 'point-of-care' para a rápida determinação de D-Dímeros
• Beneficiário: Nikola Tasic
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 18/14425-7 - Um novo rumo sinérgico para a microextração por difusão gasosa (GDME) fazendo uso de polímeros com impressão molecular (MIPs)
• Beneficiário: Luís Francisco Moreira Gonçalves
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores