Effect of passive ultrasonic activation on microorganisms in primary root canal infection: a randomized clinical trial

Autor(es)

Esteban Isai Flores Orozco ^[1] ; Cassia Cestari Toia ^[2] ; Daiana Cavalli ^[3] ; Rayana Duarte Khoury ^[4] ; Flávia Goulart da Rosa Cardoso ^[5] ; Eduardo Bresciani ^[6] ; Marcia Carneiro Valera ^[7]

Afiliação do(s) autor(es)

^[1] Universidade Estadual Paulista. Instituto de Ciências e Tecnologia. Departamento de Odontologia Restauradora - Brasil

^[2] Universidade Estadual Paulista. Instituto de Ciências e Tecnologia. Departamento de Odontologia Restauradora - Brasil

^[3] Universidade Estadual Paulista. Instituto de Ciências e Tecnologia. Departamento de Odontologia Restauradora - Brasil

^[4] Universidade Estadual Paulista. Instituto de Ciências e Tecnologia. Departamento de Odontologia Restauradora - Brasil

^[5] Universidade Estadual Paulista. Instituto de Ciências e Tecnologia. Departamento de Odontologia Restauradora - Brasil

^[6] Universidade Estadual Paulista. Instituto de Ciências e Tecnologia. Departamento de Odontologia Restauradora - Brasil

^[7] Universidade Estadual Paulista. Instituto de Ciências e Tecnologia. Departamento de Odontologia Restauradora - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Applied Oral Science; v. 28, 2019-11-28.

Resumo

Abstract Objective: This clinical study sought to evaluate the effectiveness of passive ultrasonic activation (PUA) in eliminating microorganisms in primary endodontic infection (PEI) after instrumentation of root canals using microbiological culture and checkerboard DNA-DNA hybridization. Methodology: Twenty root canals with PEI and apical periodontitis were selected. The root canals were instrumented and then randomly divided into 2 groups, according to the irrigation method: PUA and conventional needle irrigation (CNI). Microbiological samples were collected before instrumentation (S1), after instrumentation (S2) and after irrigation with 17% EDTA (S3). The samples were subjected to anaerobic culture technique and checkerboard DNA-DNA hybridization analysis. Results: A statistically significant difference was found between CNI (23.56%) and PUA (98.37%) regarding the median percentage values for culturable bacteria reduction (p<0.05). In the initial samples, the most frequently detected species was S. constellatus (50%), and after root canal treatment was E. faecalis (50%). Conclusion: Both treatments significantly decreased the number of bacterial species compared with the initial sample. However, no statistical difference in the total microbial load between PUA and CNI groups was detected. The number of cultivable anaerobic bacteria reduced significantly using PUA, and the bacterial composition and number of bacterial species after using either CNI or PUA was similar. (AU)

• Processo FAPESP: 14/25789-9 - Quantificação de endotoxinas e sua relação com sinais / sintomas e volumetria do canal radicular e da lesão periapical em dentes com infecção endodôntica primária
• Beneficiário: Marcia Carneiro Valera Garakis
• Modalidade de apoio: Auxílio à Pesquisa - Regular