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Autor(es):
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Bonifacio, Bruna Vidal
[1, 2, 3]
;
Machado Vila, Taissa Vieira
[2, 3]
;
Masiero, Isadora Fantacini
[1]
;
da Silva, Patricia Bento
[1]
;
da Silva, Isabel Cristiane
[1]
;
Lopes, Erica de Oliveira
[1]
;
dos Santos Ramos, Matheus Aparecido
[1]
;
de Souza, Leonardo Perez
[4]
;
Vilegas, Wagner
[5]
;
Pavan, Fernando Rogerio
[1]
;
Chorilli, Marlus
[1]
;
Lopez-Ribot, Jose Luis
[2, 3]
;
Bauab, Tais Maria
[1]
Número total de Autores: 13
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| Afiliação do(s) autor(es): | [1] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Araraquara, SP - Brazil
[2] Univ Texas San Antonio, Dept Biol, San Antonio, TX 78249 - USA
[3] Univ Texas San Antonio, South Texas Ctr Emerging Infect Dis, San Antonio, TX 78249 - USA
[4] Sao Paulo State Univ UNESP, Inst Chem, Araraquara, SP - Brazil
[5] Sao Paulo State Univ UNESP, Inst Biosci, Sao Vicente - Brazil
Número total de Afiliações: 5
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| Tipo de documento: |
Artigo Científico
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| Fonte: |
FRONTIERS IN MICROBIOLOGY;
v. 10,
NOV 15 2019.
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| Citações Web of Science: |
0
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| Resumo |
We have previously reported on the activity of different extracts from Astronium sp. against Candida albicans, with the hydroethanolic extract prepared from leaves of A. urundeuva, an arboreal species widely distributed in arid environments of South America and often used in folk medicine, displaying the highest in vitro activity. Here we have further evaluated the antifungal activity of this extract against strains of C. albicans and C. glabrata, the two most common etiological agents of candidiasis. The extract was tested alone and loaded into a nanostructured lipid system (10% oil phase, 10% surfactant and 80% aqueous phase, 0.5% Poloxamer 407 (R)). In vitro susceptibility assays demonstrated the antifungal activity of the free extract and the microemulsion against both Candida species, with increased activity against C. glabrata, including collection strains and clinical isolates displaying different levels of resistance against the most common clinically used antifungal drugs. Checkerboard results showed synergism when the free extract was combined with amphotericin B against C. albicans. Serial passage experiments confirmed development of resistance to fluconazole but not to the free extract upon prolonged exposure. Although preformed biofilms were intrinsically resistant to treatment with the extract, it was able to inhibit biofilm formation by C. albicans at concentrations comparable to those inhibiting planktonic growth. Cytotoxicity assays in different cell lines as well as an alternative model using Artemia salina L. confirmed a good safety profile of the both free and loaded extracts, and an in vivo assay demonstrated the efficacy of the free and loaded extracts when used topically in a rat model of vaginal candidiasis. Overall, these results reveal the promise of the A. urundeuva leaves extract to be further investigated and developed as an antifungal. (AU) |
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| Processo FAPESP: |
13/25432-0 - Atividade in vitro e in vivo anti-Candida albicans e anti-Helicobacter pylori de extratos de Astronium sp. incorporados ou não em sistemas nanoestruturados
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| Beneficiário: | Taís Maria Bauab |
| Linha de fomento: |
Auxílio à Pesquisa - Regular
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| Processo FAPESP: |
09/52237-9 - Fitoterápicos padronizados como alvo para o tratamento de doenças crônicas
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| Beneficiário: | Wagner Vilegas |
| Linha de fomento: |
Auxílio à Pesquisa - Programa BIOTA - Temático
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| Processo FAPESP: |
15/19011-8 - Extrato de Astronium urundeuva como agente antifúngico: explorando o mecanismo de ação contra Candida SP
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| Beneficiário: | Bruna Vidal Bonifácio |
| Linha de fomento: |
Bolsas no Exterior - Estágio de Pesquisa - Doutorado
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| Processo FAPESP: |
13/25121-5 - Prospecção químico-biológica de extratos hidroetanólicos das folhas de Astronium urundeuva na investigação do potencial antifúngico
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| Beneficiário: | Bruna Vidal Bonifácio |
| Linha de fomento: |
Bolsas no Brasil - Doutorado
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