Detection of Increased Relative Expression Units of Bacteroides and Prevotella, and Decreased Clostridium leptum in Stool Samples from Brazilian Rheumatoid Arthritis Patients: A Pilot Study

Autor(es)

Rodrigues, Guilherme S. P. ^[1] ; Cayres, Leonardo C. F. ^[1] ; Goncalves, Fernanda P. ^[1] ; Takaoka, Nauyta N. C. ^[1] ; Lengert, Andre H. ^[2] ; Tansini, Aline ^[2] ; Brisotti, Joao L. ^[3] ; Sasdelli, Carolina B. G. ^[3] ; de Oliveira, V, Gislane L.

Afiliação do(s) autor(es)

^[1] V, Sch Hlth Sci Dr Paulo Prata, Microbiome Study Grp, BR-14785002 Barretos, SP - Brazil

^[2] Barretos Canc Hosp, Mol Oncol Res Ctr, BR-14784400 Barretos, SP - Brazil

^[3] Barretos Med Specialties Outpatient AME, BR-14785000 Barretos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MICROORGANISMS; v. 7, n. 10 OCT 2019.
• Citações Web of Science: 1

Resumo

Interactions between gut microbes and disease modifying antirheumatic drugs (DMARDs) have been proposed. The aim of the present study was to evaluate the presence of some specific bacteria in stool samples from Brazilian RA patients receiving DMARDs and correlate these data with diet, clinical parameters, and cytokines. Stool samples were used for gut bacteria evalutation by qPCR. Serum samples were used to quantify IL-4 and IL-10 by flow cytometer. Statistics were performed by Pearson chi-square, Mann-Whitney U test, and Spearman's correlation. The study included 20 RA patients and 30 healthy controls. There were no significant differences (p > 0.05) in dietary habits between RA patients and controls. Concerning gut bacteria, we observed an increase in relative expression units (REU) of Bacteroides and Prevotella species in stool samples from patients, and a decrease in REU of Clostridium leptum when compared with healthy controls. Positive correlation between Prevotella and rheumatoid factor was detected. The IL-4 and IL-10 concentrations were increased in patients when compared with controls. We concluded that gut bacteria are different between RA patients receiving DMARDs and healthy controls. Further studies are necessary to determine the real role of gut microbes and their metabolities in clinical response to different DMARDs in RA patients. (AU)

• Processo FAPESP: 19/22090-8 - Prevalência de espécies de Bacteroides e Prevotella, e diminuição de Clostridium leptum na microbiota intestinal de pacientes com artrite reumatoide: estudo piloto
• Beneficiário: João Luiz Brisotti
• Modalidade de apoio: Auxílio à Pesquisa - Publicações científicas - Artigo
• Processo FAPESP: 17/03463-2 - Avaliação da disbiose intestinal em pacientes com artrite reumatoide e correlação com citocinas do padrão Th1 e Th17
• Beneficiário: Guilherme Siqueira Pardo Rodrigues
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica