CTX-M-producing Escherichia coli Isolated from urban pigeons (Columba livia domestica) in Brazil

Autor(es)

Cunha, Marcos P. V. ^[1] ; Oliveira, Mirela C. V. ^[1] ; Oliveira, Maria G. X. ^{[1, 2]} ; Menao, Marcia C. ^[2] ; Knobl, Terezinha ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Pathol, Av Prof Dr Orlando Marques de Paiva 87, BR-05508270 Sao Paulo, SP - Brazil

^[2] FMU, Sch Vet, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF INFECTION IN DEVELOPING COUNTRIES; v. 13, n. 11, p. 1052-1056, NOV 2019.
• Citações Web of Science: 0

Resumo

Introduction: Worldwide urban pigeons (Columba livia domestica) arc an important reservoir of pathogenic and multidrug-resistant bacteria (MDR). Plasmids are key genetic elements in the dissemination of antimicrobial drug resistance in bacteria, including beta-lactams and quinolones, which are the most important classes of drugs for treatment of Enterobacteriaceae infections in human and veterinary medicine. The aim of this study was to determine the presence of Escherichia coli (E. coli) harboring plasmids containing extend-spectrum (ESBL) and pAmpC beta-lactamases, also plasmid-mediated quinolone resistance (PMQR) genes in urban pigeons from Sao Paulo State, Brazil. Methodology: A collection of 107 isolates of E. coli from urban pigeons from four cities was screened by antimicrobial resistance phenotypic and PCR for genes encoding ESBL, pAmpC and PMQR genes. Clonality was evaluated by ERIC-PCR. Results: We found three strains positive for bla(CTX-M) genes. In two clonally related CTX-M-8-producing strains, the gene was associated with IncI1 plasmids. An MDR strain harboring bla(CTX-M-2) the plasmid could not be transferred. No strain was positive for PMQR genes. Conclusion: These results indicate that CTX-M-2 and CTX-M-8-producing E. coli are present in urban pigeons, which could serve as a reservoir for ESBL-producing E. coli in Brazil. (AU)

• Processo FAPESP: 16/12641-9 - Resistência Antimicrobiana de Escherichia coli isoladas de aves criadas em sistemas de produção convencionais e orgânicos.
• Beneficiário: Terezinha Knöbl
• Modalidade de apoio: Auxílio à Pesquisa - Regular