Incorporating N-acetylcysteine and tricalcium phosphate into epoxy resin-based sealer improved its biocompatibility and adhesiveness to radicular dentine

Autor(es)

Ribeiro Camargo, Carlos Henrique ^{[1, 2]} ; Landim Gomes, Lais Carolina ^[2] ; Moreira Franca, Monique Costa ^[2] ; Bittencourt, Tatiane Sampaio ^[2] ; Valera, Marcia Carneiro ^[2] ; Camargo, Samira Esteves Afonso ^[1] ; Bottino, Marco Cicero ^[3]

Afiliação do(s) autor(es)

^[1] Univ Florida, Coll Dent, Dept Restorat Dent Sci, Gainesville, FL - USA

^[2] Sao Paulo State Univ, Sch Dent, Dept Restorat Dent, Sao Jose Dos Campos, SP - Brazil

^[3] Univ Michigan, Sch Dent, Dept Cariol Restorat Sci & Endodont, Ann Arbor, MI 48109 - USA

• Tipo de documento: Artigo Científico
• Fonte: Dental Materials; v. 35, n. 12, p. 1750-1756, DEC 2019.
• Citações Web of Science: 0

Resumo

Objective. This in vitro study was designed to evaluate the biocompatibility, adhesiveness, and antimicrobial activity of epoxy resin-based sealer associated with N-Acetylcysteine (NAC) or beta-tricalcium phosphate nanoparticles (beta-TCP) as an experimental retro-filling material. Methods. Cytotoxicity was assessed using 2,3-Bis-(Methoxy-4-Nitro-5-Sulphophenyl)-2H- Tetrazolium-5-Carboxanilide (XTT) and Sulforhodamine B (SRB) assays after exposing human periodontal ligament fibroblasts to extracts of the materials for 1, 3, or 7 days. For the adhesive resistance test, root canals (48 single-root teeth) were instrumented with Reciproc \#40 files (VDW GmbH, Germany) and obturated. After 7 days, the apices were sectioned and a retrograde cavity prepared and filled with the experimental materials (Mineral trioxide aggregate, Epoxy sealer, Epoxy sealer + NAC, and Epoxy sealer+ beta-TCP). For the push-out test, one 2-mm thick slice was obtained from the apical third of each specimen. Antimicrobial activity was performed using agar diffusion method. Biofilms were grown in microplates and exposed to the extracts of retro-filled materials, followed by analysis of growth inhibition on agar plates. Results. Epoxy sealer in association with beta-TCP or NAC showed better bond strength while Mineral trioxide aggregate allowed for the lowest adhesion. Mineral trioxide aggregate, Epoxy sealer+ beta-TCP, and Epoxy sealer + NAC showed low cytotoxicity. Epoxy sealer was the most cytotoxic. In antimicrobial activity assays, all materials had no effect on Candida albicans. Addition of NAC improved the antimicrobial property of Epoxy sealer against Enterococcus faecalis compared to unmodified Epoxy sealer (P < 0.05). Significance. Incorporating beta-TCP or NAC with Epoxy sealer could improve the adhesiveness and biocompatibility for better use in endodontic therapy. (C) 2019 The Academy of Dental Materials. Published by Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 15/23324-1 - Propriedades citotóxicas, antimicrobianas e adesivas da adição de N-acetilcisteína e de tricálcio fosfato a um cimento endodôntico à base de resina epóxi, para o uso em retro-obturação
• Beneficiário: Laís Carolina Landim Gomes
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica