Effects of pentoxifylline supplementation to semen extender on post-breeding inflammation response assessed by endometrial cytology and vascular perfusion in mares

Autor(es)

Tsunoda, Roberta Harue ^[1] ; Serrano-Recalde, Elena Carolina ^[1] ; de Arruda, Rubens Paes ^[2] ; Martins Oliveira, Bruna Marcele ^[1] ; Costa Pinto, Samara Cristine ^[1] ; Carvalho Celeghini, Eneiva Carla ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ctr Biotechnol Anim Reprod, Dept Anim Reprod, Lab Teaching & Res Pathol Reprod, Sch Vet Med & An, Pirassununga, SP - Brazil

^[2] Univ Sao Paulo, Sch Vet Med & Anim Sci, Ctr Biotechnol Anim Reprod, Dept Anim Reprod, Lab Semen Biotechnol & Androl, Pirassununga, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Animal Reproduction Science; v. 208, SEP 2019.
• Citações Web of Science: 0

Resumo

The aim was to evaluate effects of addition of pentoxifylline to skimmed milk semen extender on uterine inflammatory response. Thirty-six estrous cycles of 15 mares were randomly divided into five groups for artificial insemination (AI): Control: mimicking the AI procedure (n = 7); Extender: deposition of skimmed milk based extender (n = 7); Extender + PTX: skimmed milk based extender plus pentoxifylline (7.18 mM; n = 8); Semen: semen diluted with extender without pentoxifylline (n = 7), and Semen + PTX: semen diluted with extender containing pentoxifylline (n = 7). Mares in estrus were examined by trans-rectal palpation and using ultrasonography, and ovulation was induced. Uterine hemodynamics were assessed immediately before ovulation induction (T-30), immediately before AI (T0), 2 (T2), 6 (T6), 12 (T12), 24 (T24) and 48 (T48) h after AI. Endometrial samples were collected 6 h after AI, and slides were stained and examined to determine percentage of PMN. Pentoxifylline had no additional effect on vascular perfusion. There was a major inflammatory response with pentoxifylline treatment that was greater than that of the control group. In the group treated with Extender + PTX, there were more PMN (57.98 +/- 9.42%) than in the group treated with Extender (20.20 +/- 6.63%) and in the Semen + PTX group more PMN (82.84 +/- 5.71%) than in the Semen-treated group (47.83 +/- 10.61%). These findings indicate the addition of pentoxifylline does not stimulate blood flow; however, it induces a greater immune defense response because more neutrophils migrate to the uterine lumen. (AU)

• Processo FAPESP: 12/14927-6 - Efeito da pentoxifilina sobre a qualidade espermática, hemodinâmica uterina e fertilidade em equinos
• Beneficiário: Roberta Harue Tsunoda
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 12/15736-0 - Taxa de prenhez, resposta inflamatória e hemodinâmica uterina influenciadas pela pentoxifilina e qualidade seminal em equinos
• Beneficiário: Eneiva Carla Carvalho Celeghini
• Modalidade de apoio: Auxílio à Pesquisa - Regular