Effects of pentoxifylline supplementation to semen extender on post-breeding inflammation response assessed by endometrial cytology and vascular perfusion in mares

Author(s)

Tsunoda, Roberta Harue ^[1] ; Serrano-Recalde, Elena Carolina ^[1] ; de Arruda, Rubens Paes ^[2] ; Martins Oliveira, Bruna Marcele ^[1] ; Costa Pinto, Samara Cristine ^[1] ; Carvalho Celeghini, Eneiva Carla ^[1]

Affiliation

^[1] Univ Sao Paulo, Ctr Biotechnol Anim Reprod, Dept Anim Reprod, Lab Teaching & Res Pathol Reprod, Sch Vet Med & An, Pirassununga, SP - Brazil

^[2] Univ Sao Paulo, Sch Vet Med & Anim Sci, Ctr Biotechnol Anim Reprod, Dept Anim Reprod, Lab Semen Biotechnol & Androl, Pirassununga, SP - Brazil

• Document type: Journal article
• Source: Animal Reproduction Science; v. 208, SEP 2019.
• Web of Science Citations: 0

Abstract

The aim was to evaluate effects of addition of pentoxifylline to skimmed milk semen extender on uterine inflammatory response. Thirty-six estrous cycles of 15 mares were randomly divided into five groups for artificial insemination (AI): Control: mimicking the AI procedure (n = 7); Extender: deposition of skimmed milk based extender (n = 7); Extender + PTX: skimmed milk based extender plus pentoxifylline (7.18 mM; n = 8); Semen: semen diluted with extender without pentoxifylline (n = 7), and Semen + PTX: semen diluted with extender containing pentoxifylline (n = 7). Mares in estrus were examined by trans-rectal palpation and using ultrasonography, and ovulation was induced. Uterine hemodynamics were assessed immediately before ovulation induction (T-30), immediately before AI (T0), 2 (T2), 6 (T6), 12 (T12), 24 (T24) and 48 (T48) h after AI. Endometrial samples were collected 6 h after AI, and slides were stained and examined to determine percentage of PMN. Pentoxifylline had no additional effect on vascular perfusion. There was a major inflammatory response with pentoxifylline treatment that was greater than that of the control group. In the group treated with Extender + PTX, there were more PMN (57.98 +/- 9.42%) than in the group treated with Extender (20.20 +/- 6.63%) and in the Semen + PTX group more PMN (82.84 +/- 5.71%) than in the Semen-treated group (47.83 +/- 10.61%). These findings indicate the addition of pentoxifylline does not stimulate blood flow; however, it induces a greater immune defense response because more neutrophils migrate to the uterine lumen. (AU)

• FAPESP's process: 12/14927-6 - Pregnancy rate, inflamatory response and uterine blood flow affected by the effect of Pentoxifylline and sêmen quality assessed by fluorecent probes
• Grantee: Roberta Harue Tsunoda
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 12/15736-0 - Pregnancy rate, inflammatory response and uterine blood flow affected by pentoxifylline and semen quality assessed in equines
• Grantee: Eneiva Carla Carvalho Celeghini
• Support Opportunities: Regular Research Grants