Persistent endometritis after breeding is a major cause of reduced fertility in mares. Recent studies have shown the importance of immunomodulation of inflammation after breeding. The aim of this study is to assess the expression of inflammatory mediators in uterus before and after artificial insemination (AI), with the purpose to verify the stimulus that semen can cause to the uterine inflammatory response, and besides, to propose a new treatment using Platelet Rich Plasma (PRP), which has been used due to its anti-inflammatory propriety.15 resistant mares and 15 susceptible to endometritis will be selected based on their reproductive history and ultrasound and cytology evaluation. Endometrial mRNA expression of the pro-inflammatory cytokines interleukin-1² (IL-1²), IL-6, IL-8 and anti-inflammatory IL-10, the determination of nitric oxide (NO) and concentrations of neutrophils in uterine secretions and endometrial cytology will be analyzed in both groups of animals; the first cycle will not have influence of medicines, and in the second the mares will be treated with 50 mL of PRP administrated inside the uterus 4 hours after AI. The expressions of all mediators will be assessed (1) in basal conditions and (2) 24 hours after AI with fresh semen. The expression of IL will be quantitated by real time PCR, the determination of NO will be done by colorimetric reaction of Griess with spectrophotometer and the concentration of neutrophils in uterine secretion will be quantified using Neubauer chamber. It is expected that the treatment with PRP must be an efficient way to immunomodulate the inflammatory response, as well as improve the resolution of inflammation that settle in uterus immediately and persists in determined mares after mate or AI.
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