ENDOMETRIAL EXPRESSION OF INFLAMMATORY INTERLEUKINS AND PROTEOMICS OF ENDOMETRIAL FLUID OF MARES SUSCEPTIBLE TO PERSISTENT BREEDING-INDUCED ENDOMETRITIS TREATED WITH PLASMA RICH PLASMA

• Grant number: 17/13883-9
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: January 01, 2018
• End date: March 13, 2021
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Marco Antonio Alvarenga
• Grantee: Lorenzo Garrido Teixeira Martini Segabinazzi
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated scholarship(s): 19/05017-5 - Endometrial expression of inflammatory cytokines and proteomics of endometrial fluid of susceptible mares to persistent breeding-induced endometritis treated with platelet-rich plasma, BE.EP.DR

Abstract

In the horse market the offspring of proven breeders are more valued, however this usually happens when mares already have advanced age and progeny proven on the competitions. This encourages to maintenance of these animals, even with low reproductive rates, in reproductive programs. In this context persistent breeding-induced endometritis (PBIE) has important impacts, being considered the third most frequent disease affecting horses and the main cause of subfertility in this species. Studies using immunomodulatory drugs have shown good results in reducing the inflammatory process, as well as increasing the fertility of these animals. More recently, the use of PRP has proved to be beneficial in the treatment of PBIE, increasing conception rates. The PRP is already widely used in veterinary medicine, in the treatment of the most diverse affections, mainly tendinous, osteoarticular, ligamentary and cutaneous lesions, as it presents mitogenic, chemotactic, neovascular and anti-inflammatory action. In this context, the present study aims to evaluate the endometrial expression of inflammatory interleukins of mares treated with PRP, as well as the proteomics of the endometrial fluid of these animals. In our laboratory, it has been observed that PRP is beneficial in the treatment of susceptible mares to PBIE, reducing the uterine inflammatory process, decreasing the number of neutrophils and COX-2 expression in the endometrium of these mares. However, further studies on the use of PRP in the treatment of mares susceptible to PBIE are necessary for the real knowledge of the action of this agent in the endometrial inflammatory process.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SEGABINAZZI, LORENZO GARRIDO TEIXEIRA M.; DA CUNHA SCHEEREN, VERONICA FLORES; FREITAS-DELL'AQUA, CAMILA DE PAULA; PAPA, FREDERICO OZANAM; ALVARENGA, MARCO ANTONIO; DELL'AQUA, JOSE ANTONIO; CANISSO, IGOR FREDERICO. Cholesterol-Loaded Cyclodextrin Addition to Skim Milk-Based Extender Enhances Donkey Semen Cooling and Fertility in Horse Mares. Journal of Equine Veterinary Science, v. 105, OCT 2021. (17/13883-9)

SEGABINAZZI, LORENZO G. T. M.; ANDRADE JR, LUIZ R. P.; ALVARENGA, MARCO A.; DELL'AQUA JR, JOSE A.; CANISSO, IGOR F.. Use of Intravaginal Progesterone-Releasing Device Results in Similar Pregnancy Rates and Losses to Long-Acting Progesterone to Synchronize Acyclic Embryo Recipient Mares. VETERINARY SCIENCES, v. 8, n. 9, SEP 2021. (17/13883-9)

GOBATO, MARIANA L. M.; SEGABINAZZI, LORENZO G. T. M.; SCHEEREN, VERONICA F. C.; BANDEIRA, RAFAEL S.; FREITAS-DELL'AQUA, CAMILA P.; DELL'AQUA JR, JOSE A.; PAPA, FREDERICO O.. Ability of donkey sperm to tolerate cooling: Effect of extender base and removal of seminal plasma on sperm parameters and fertility rates in mares. FRONTIERS IN VETERINARY SCIENCE, v. 9, p. 12-pg., 2022-09-26. (17/13883-9)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SEGABINAZZI, Lorenzo Garrido Teixeira Martini. Modulation of uterine inflammatory response in mares treated with platelet-rich plasma.. 2021. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu Botucatu.