Study of proteins with mercury in fish from the Amazon region

Autor(es)

Cavecci-Mendonca, Bruna ^[1] ; de Souza Vieira, Jose Cavalcante ^[1] ; de Lima, Paula Monteiro ^[1] ; Leite, Aline Lima ^[2] ; Rabelo Buzalaf, Marilia Afonso ^[2] ; Zara, Luiz Fabricio ^[3] ; Padilha, Pedro de Magalhaes ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ Julio de Mesquita Filho, Dept Chem & Biochem, Botucatu, SP - Brazil

^[2] Univ Sao Paulo, Bauru Fac Dent, Sao Paulo, SP - Brazil

^[3] Univ Brasilia, Brasilia, DF - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Food Chemistry; v. 309, MAR 20 2020.
• Citações Web of Science: 0

Resumo

The high concentrations of mercury found in Amazon have been intensively studied by the scientific community in the last decades. These mercurial species bind preferentially to proteins. Therefore, this work proposal sought to obtain the fractionation, identification and study of mercury - bound proteins present in samples of muscular and hepatic tissue from fish collected in the reservoir of the Jirau Hydroelectric Power Plant - on the Madeira River. Two-dimensional electrophoresis (2D-PAGE) for protein fractionation, graphite furnace atomic absorption spectrometry (GFAAS) for the quantification of mercury and Mass Spectrometry (ESI-MS/MS) were used for the identification of proteins. Concluding the work with analysis of graphics from the Blast2go program. Two mercury - bound proteins were identified as triosephosphate isomerase A and Protein FAM45A. The data generated by the bioinformatics programs confirm the tendency of these proteins to be linked to mercury and elucity the possibles existing physiological and cellular interactions. (AU)

• Processo FAPESP: 14/02638-5 - Estudo de metalotioneínas associadas ao mercúrio em peixes da região amazônica
• Beneficiário: Bruna Cavecci Mendonça
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 16/19404-2 - Caracterização de biomarcadores de toxicidade de mercúrio em peixes da Bacia do Rio Madeira-Rondônia utilizando-se estratégias metaloproteômicas
• Beneficiário: Pedro de Magalhães Padilha
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/21297-1 - Metalotioneínas em peixes da região amazônica: estudo metalômico associado ao mercúrio
• Beneficiário: Pedro de Magalhães Padilha
• Linha de fomento: Auxílio à Pesquisa - Regular