Study of proteins with mercury in fish from the Amazon region

Author(s)

Cavecci-Mendonca, Bruna ^[1] ; de Souza Vieira, Jose Cavalcante ^[1] ; de Lima, Paula Monteiro ^[1] ; Leite, Aline Lima ^[2] ; Rabelo Buzalaf, Marilia Afonso ^[2] ; Zara, Luiz Fabricio ^[3] ; Padilha, Pedro de Magalhaes ^[1]

Affiliation

^[1] Sao Paulo State Univ Julio de Mesquita Filho, Dept Chem & Biochem, Botucatu, SP - Brazil

^[2] Univ Sao Paulo, Bauru Fac Dent, Sao Paulo, SP - Brazil

^[3] Univ Brasilia, Brasilia, DF - Brazil

• Document type: Journal article
• Source: Food Chemistry; v. 309, MAR 20 2020.
• Web of Science Citations: 0

Abstract

The high concentrations of mercury found in Amazon have been intensively studied by the scientific community in the last decades. These mercurial species bind preferentially to proteins. Therefore, this work proposal sought to obtain the fractionation, identification and study of mercury - bound proteins present in samples of muscular and hepatic tissue from fish collected in the reservoir of the Jirau Hydroelectric Power Plant - on the Madeira River. Two-dimensional electrophoresis (2D-PAGE) for protein fractionation, graphite furnace atomic absorption spectrometry (GFAAS) for the quantification of mercury and Mass Spectrometry (ESI-MS/MS) were used for the identification of proteins. Concluding the work with analysis of graphics from the Blast2go program. Two mercury - bound proteins were identified as triosephosphate isomerase A and Protein FAM45A. The data generated by the bioinformatics programs confirm the tendency of these proteins to be linked to mercury and elucity the possibles existing physiological and cellular interactions. (AU)

• FAPESP's process: 14/02638-5 - Study metallothionein associated with mercury in fish of the Amazon Region
• Grantee: Bruna Cavecci Mendonça
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 16/19404-2 - Mercury toxicity biomarker characterization in fish from Basin of the Madeira River-Rondônia using metalloproteomic strategies
• Grantee: Pedro de Magalhães Padilha
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/21297-1 - Metallothioneins in fish from Amazon Region: metallomic study associated to mercury
• Grantee: Pedro de Magalhães Padilha
• Support Opportunities: Regular Research Grants