Effect of beta-alanine supplementation during high-intensity interval training on repeated sprint ability performance and neuromuscular fatigue

Autor(es)

Milioni, Fabio ^[1] ; Bonetti de Poli, Rodrigo Araujo ^[1] ; Saunders, Bryan ^{[2, 3]} ; Gualano, Bruno ^[2] ; da Rocha, Alisson L. ^[4] ; Ramos da Silva, Adelino Sanchez ^[4] ; Guerrero Muller, Paulo de Tarso ^[5] ; Zagatto, Alessandro Moura ^{[1, 6]}

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Lab Physiol & Human Performance, Post Grad Program Human Movement Sci, Bauru, SP - Brazil

^[2] Univ Sao Paulo, Fac Med, Sch Phys Educ & Sport, Rheumatol Div, Appl Physiol & Nutr Res Grp, Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Fac Med, Inst Orthopaed & Traumatol, Ribeirao Preto, SP - Brazil

^[4] Univ Sao Paulo, Sch Phys Educ & Sports Ribeirao Preto, Ribeirao Preto, SP - Brazil

^[5] Mato Grosso do Sul Fed Univ, Lab Resp Pathophysiol, Sao Paulo, SP - Brazil

^[6] Sao Paulo State Univ, Fac Sci, Dept Phys Educ, Bauru, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Applied Physiology; v. 127, n. 6, p. 1599-1610, DEC 2019.
• Citações Web of Science: 1

Resumo

The study investigated the influence of beta-alanine supplementation during a high-intensity interval training (HIIT) program on repeated sprint ability (RSA) performance. This study was randomized, double-blinded, and placebo controlled. Eighteen men performed an incremental running test until exhaustion (T-INC) at baseline and followed by 4-wk HIIT (10 x 1min runs 90% maximal T-INC velocity {[}1-min recovery]). Then, participants were randomized into two groups and performed a 6-wk HIIT associated with supplementation of 6.4 g/day of beta-alanine (G beta) or dextrose (placebo group; GP). Pre- and post-6-wk HIIT + supplementation, participants performed the following tests: 1) T-INC; 2) supramaximal running test; and 3) 2 x 6 x 35-m sprints (RSA). Before and immediately after RSA, neuromuscular function was assessed by vertical jumps, maximal isometric voluntary contractions of knee extension, and neuromuscular electrical stimulations. Muscle biopsies were performed to determine muscle carnosine content, muscle buffering capacity in vitro (beta m(in) (vitro)), and content of phos-phofructokinase (PFK), monocarboxylate transporter 4 (MCT4), and hypoxia-inducible factor-1 alpha (HIF-1 alpha). Both groups showed a significant time effect for maximal oxygen uptake (G beta: 6.2 +/- 3.6% and GP: 6.5 +/- 4.2%; P > 0.01); only G beta showed a time effect for total (-3.0 +/- 2.0%; P > 0.001) and best (-3.3 +/- 3.0%; P = 0.03) RSA times. A group-by-time interaction was shown after HIIT + Supplementation for muscle carnosine (G beta: 34.4 +/- 2.3 mmol.kg(-1).dm(-1) and GP: 20.7 +/- 3.0 mmol.kg(-1) dm(-1); P = 0.003) and neuromuscular voluntary activation after RSA (G beta: 87.2 +/- 3.3% and GP: 78.9 +/- 12.4%; P = 0.02). No time effect or group-by-time interaction was shown for supramaximal running test performance, beta m, and content of PFK, MCT4, and HIF-1 alpha. In summary, beta-alanine supplementation during HIIT increased muscle carnosine and attenuated neuromuscular fatigue, which may contribute to an enhancement of RSA performance. NEW \& NOTEWORTHY beta-Alanine supplementation during a high-intensity interval training program increased repeated sprint performance. The improvement of muscle carnosine content induced by beta-alanine supplementation may have contributed to an attenuation of central fatigue during repeated sprint. Overall, beta-alanine supplementation may be a useful dietary intervention to prevent fatigue. (AU)

• Processo FAPESP: 16/11076-6 - Suplementação de ²-alanina associado ao treinamento intervalado de alta intensidade no desempenho e na manifestação dos processos de fadiga central e periférica durante sprints repetidos
• Beneficiário: Alessandro Moura Zagatto
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/50438-0 - Suplementação nutricional e exercício para otimizar o desempenho: foco nas respostas individuais e um passo para a nutrição esportiva personalizada
• Beneficiário: Bryan Saunders
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 16/17836-2 - Efeitos da pós ativação neuromuscular induzida por saltos na capacidade anaeróbia em cicloergômetro
• Beneficiário: Rodrigo de Araujo Bonetti de Poli
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 16/02683-6 - Influência da suplementação de beta-alanina associado ao treinamento intervalado de alta intensidade no desempenho de sprints repetidos.
• Beneficiário: Fabio Milioni
• Modalidade de apoio: Bolsas no Brasil - Doutorado