Miniemulsion in biocatalysis, a new approach employing a solid reagent and an easy protocol for product isolation applied to the aldol reaction by Rhizopus niveus lipase

Autor(es)

Birolli, Willian G. ^{[1, 2]} ; Porto, Andre L. M. ^[1] ; Fonseca, Luis P. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sao Carlos Inst Chem, Ave Joao Dagnone 1100, Ed Quim Ambiental, BR-13563120 Sao Carlos, SP - Brazil

^[2] Univ Lisbon, Inst Super Tecn, Inst Bioengn & Biosci, Bioengn Dept, Ave Rovisco Pais, P-1049001 Lisbon - Portugal

• Tipo de documento: Artigo Científico
• Fonte: Bioresource Technology; v. 297, FEB 2020.
• Citações Web of Science: 0

Resumo

Miniemulsion systems presented a great potential for biocatalytic reactions. However, different limitations jeopardized the applications of this non-conventional medium. In this work, miniemulsion systems (emulsion reactors) were applied for the first time to the aldol reaction between cyclohexanone and 4-nitrobenzaldehyde by Rhizopus niveus lipase allowing a decrease in the catalyst concentration from 20 mg mL(-1) to 6 mg mL(-1) in comparison with organic solvents. Moreover, the yield increased from similar to 25% to similar to 65% for 48 h reactions and the enantiomeric excess increased from similar to 10% to similar to 30% for (R,S)-anti-aldol product, showing the potentiality of this non-conventional reaction medium. The advances reported in this work expands the possibilities of the miniemulsion reaction medium to a whole new level, increasing the scope to solid reagents and products, and also different reactions (biocatalytic or not) that requires pH control by buffers with a simple product isolation procedure, enabling future applications of this poorly studied reaction medium. (AU)

• Processo FAPESP: 14/18257-0 - Novas abordagens em reações biocatalíticas
• Beneficiário: Andre Luiz Meleiro Porto
• Modalidade de apoio: Auxílio à Pesquisa - Regular