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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Miniemulsion in biocatalysis, a new approach employing a solid reagent and an easy protocol for product isolation applied to the aldol reaction by Rhizopus niveus lipase

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Author(s):
Birolli, Willian G. [1, 2] ; Porto, Andre L. M. [1] ; Fonseca, Luis P. [2]
Total Authors: 3
Affiliation:
[1] Univ Sao Paulo, Sao Carlos Inst Chem, Ave Joao Dagnone 1100, Ed Quim Ambiental, BR-13563120 Sao Carlos, SP - Brazil
[2] Univ Lisbon, Inst Super Tecn, Inst Bioengn & Biosci, Bioengn Dept, Ave Rovisco Pais, P-1049001 Lisbon - Portugal
Total Affiliations: 2
Document type: Journal article
Source: Bioresource Technology; v. 297, FEB 2020.
Web of Science Citations: 0
Abstract

Miniemulsion systems presented a great potential for biocatalytic reactions. However, different limitations jeopardized the applications of this non-conventional medium. In this work, miniemulsion systems (emulsion reactors) were applied for the first time to the aldol reaction between cyclohexanone and 4-nitrobenzaldehyde by Rhizopus niveus lipase allowing a decrease in the catalyst concentration from 20 mg mL(-1) to 6 mg mL(-1) in comparison with organic solvents. Moreover, the yield increased from similar to 25% to similar to 65% for 48 h reactions and the enantiomeric excess increased from similar to 10% to similar to 30% for (R,S)-anti-aldol product, showing the potentiality of this non-conventional reaction medium. The advances reported in this work expands the possibilities of the miniemulsion reaction medium to a whole new level, increasing the scope to solid reagents and products, and also different reactions (biocatalytic or not) that requires pH control by buffers with a simple product isolation procedure, enabling future applications of this poorly studied reaction medium. (AU)

FAPESP's process: 14/18257-0 - New approaches in biocatalytic reactions
Grantee:Andre Luiz Meleiro Porto
Support type: Regular Research Grants