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Ultrasound application in the hydrolysis of microalgal oil catalyzed by lipases from different sources

Grant number: 19/18064-1
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): November 01, 2019
Effective date (End): October 31, 2020
Field of knowledge:Biological Sciences - Microbiology
Principal Investigator:Patricia Caroline Molgero da Rós
Grantee:Fellipe Cesar Cardoso de Souza Felix
Home Institution: Escola de Engenharia de Lorena (EEL). Universidade de São Paulo (USP). Lorena , SP, Brazil

Abstract

The application of unconventional heating methods to carry enzymatic reactions has been proved primordial in substitute traditional processes. The enzymatic hydrolysis of oils and fats is a technological process that allows the production of fatty acids with high added value (PUFAs) and low energy use. Lipases are enzymes of vegetal, animal or microbial origin, which catalyze total or partial hydrolysis of oils and fats. This way, this project has the aim to verify the catalytic action of lipases from different sources, especially lipases from Candida rugosa, Rhizopus oryzae and pancreatic in the hydrolysis reaction of microalgal oil employing unconventional heating methods (ultrasound), as well as optimize reactions parameters through a study that allows verify the influence of the meaning variables that might chances the reaction efficiency such as: ultrasound potency and temperature. A 22 full factorial design will be carried for this purpose, simultaneously evaluating the power of the ultrasound (40, 70 e 100) and temperature (40, 45 e 50 °C) in enzymatic hydrolysis percent of microalgal oil. The main aspects that will be studied in this work include microalgae cultivation in bubble column photobioreactors, biomass harvesting and recovery, extraction of microbial oil and finally, the extracted oil will be used as substrate in the enzymatic hydrolysis reactions employing ultrasound as heating source.