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Agroindustrial residues as indirect and direct sources of lipases: obtaining at low cost, applications in hydrolysis and synthesis reactions and structural correlations with biological activities

Grant number: 15/01753-8
Support type:Regular Research Grants
Duration: July 01, 2015 - June 30, 2017
Field of knowledge:Biological Sciences - Botany
Principal researcher:Luciana Francisco Fleuri
Grantee:Luciana Francisco Fleuri
Home Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
Assoc. researchers:Willian Fernando Zambuzzi

Abstract

The agroindustrial residues are considered a world-wide problem and they can be used as substrates for solid state fermentation (SSF) processes aiming the achievement of fungal lipases (indirectly), or they can be the sources of such enzymes (direct form). The lipases are capable of catalyzing the hydrolysis of glycerides, as well as several synthesis reactions in micro-aqueous conditions. However, its use is still limited, mainly due to high cost of these enzymes, which are market for high values and the small number of industries that hold this technology. So lipases produced by indirectly or directly from these residues may contribute to lower the production cost of these biocatalysts allowing different applications. In addition, the products of lipases hydrolysis and synthesis may also show activities such as antioxidant, antimicrobial and antitumor, which expand its use. In the light of these statements, fungi will be used for SSF for lipase production using new residues (dried distillers grains with solubles and waste residues from the orange process), followed by optimization of the fermentation conditions. The most promising enzymes (fungal and vegetable) will be characterized as for specificity to fatty acids of different sizes of carbon chain and regionselectivity. Then, lipases will be up scale produced/obtained, concentrated and applied in hydrolysis reactions aiming the bioremediation; esterification and alcoholysis for biodiesel production and interesterification for modification of oils and fats. The products of these reactions will also be tested for several biological activities, which will be correlated with chemical structure and / or components responsible for these activities. (AU)

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Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BENELLI FRANCISCO, VALESCA CRISTIANE; OKINO-DELGADO, CLARISSA HAMAIO; ZANUTTO ELGUI, MIRELLA R.; DA COSTA FERNANDES, CELIO JUNIOR; FACANALI, ROSELAINE; DA SILVA, RODRIGO AUGUSTO; ZAMBUZZI, WILLIAN FERNANDO; MAYO MARQUES, MARCIA ORTIZ; FLEURI, LUCIANA FRANCISCO. Plant oil bioconversion into increase biological activity through lipases derived from wastes. INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, v. 54, n. 4, SI, p. 1089-1099, APR 2019. Web of Science Citations: 0.
OKINO-DELGADO, CLARISSA HAMAIO; PEREIRA, MILENE STEFANI; INACIO DA SILVA, JOAO VITOR; KHARFAN, DANIELA; DO PRADO, DEBORA ZANONI; FLEURI, LUCIANA FRANCISCO. Lipases obtained from orange wastes: Commercialization potential and biochemical properties of different varieties and fractions. BIOTECHNOLOGY PROGRESS, v. 35, n. 1 JAN 2019. Web of Science Citations: 0.
ATHANAZIO-HELIODORO, JULIA CAROLINA; OKINO-DELGADO, CLARISSA HAMAIO; DA COSTA FERNANDES, CELIO JUNIOR; ZANUTTO, MIRELLA ROSSITTO; DO PRADO, DEBORA ZANONI; DA SILVA, RODRIGO AUGUSTO; FACANALI, ROSELAINE; ZAMBUZZI, WILLIAN FERNANDO; MAYO MARQUES, MARCIA ORTIZ; FLEURI, LUCIANA FRANCISCO. Improvement of lipase obtaining system by orange waste-based solid-state fermentation: production, characterization and application. PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, v. 48, n. 7, p. 565-573, 2018. Web of Science Citations: 3.
OKINO-DELGADO, CLARISSA HAMAIO; DO PRADO, DEBORA ZANONI; FACANALI, ROSELAINE; ORTIZ MARQUES, MARCIA MAYO; NASCIMENTO, AUGUSTO SANTANA; DA COSTA FERNANDES, CELIO JUNIOR; ZAMBUZZI, WILLIAM FERNANDO; FLEURI, LUCIANA FRANCISCO. Bioremediation of cooking oil waste using lipases from wastes. PLoS One, v. 12, n. 10 OCT 26 2017. Web of Science Citations: 10.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.