The Pneumatron: An automated pneumatic apparatus for estimating xylem vulnerability to embolism at high temporal resolutionKey-words

Autor(es):Mostrar menos -

Pereira, Luciano ^{[1, 2]} ; Bittencourt, Paulo R. L. ; Pacheco, Vinicius S. ^[3] ; Miranda, Marcela T. ^[3] ; Zhang, Ya ; Oliveira, Rafael S. ; Groenendijk, Peter ; Machado, Eduardo C. ^[1] ; Tyree, Melvin T. ^[4] ; Jansen, Steven ^[5] ; Rowland, Lucy ^[6] ; Ribeiro, Rafael V. ^[2]

Afiliação do(s) autor(es)

^[1] Agron Inst IAC, Ctr R&D Ecophysiol & Biophys, Lab Plant Physiol Coaracy M Franco, Campinas, SP - Brazil

^[2] Univ Campinas UNICAMP, Dept Plant Biol, Inst Biol, Lab Crop Physiol, POB 6109, BR-13083970 Campinas, SP - Brazil

^[3] Univ Estadual Campinas, Inst Biol, Dept Plant Biol, POB 6109, BR-13083970 Campinas, SP - Brazil

^[4] Zhejiang Normal Univ, Coll Chem & Life Sci, Jinhua 321004, Zhejiang - Peoples R China

^[5] Univ Ulm, Inst Systemat Bot & Ecol, D-89081 Ulm - Germany

^[6] Univ Exeter, Coll Life & Environm Sci, Exeter, Devon - England

• Tipo de documento: Artigo Científico
• Fonte: PLANT CELL AND ENVIRONMENT; v. 43, n. 1, p. 131-142, JAN 2020.
• Citações Web of Science: 0

Resumo

Xylem vulnerability to embolism represents an important trait to determine species distribution patterns and drought resistance. However, estimating embolism resistance frequently requires time-consuming and ambiguous hydraulic lab measurements. Based on a recently developed pneumatic method, we present and test the ``Pneumatron{''}, a device that generates high time-resolution and fully automated vulnerability curves. Embolism resistance is estimated by applying a partial vacuum to extract air from an excised xylem sample, while monitoring the pressure change over time. Although the amount of gas extracted is strongly correlated with the percentage loss of xylem conductivity, validation of the Pneumatron was performed by comparison with the optical method for Eucalyptus camaldulensis leaves. The Pneumatron improved the precision of the pneumatic method considerably, facilitating the detection of small differences in the (percentage of air discharged {[}PAD] < 0.47%). Hence, the Pneumatron can directly measure the 50% PAD without any fitting of vulnerability curves. PAD and embolism frequency based on the optical method were strongly correlated (r(2) = 0.93) for E. camaldulensis. By providing an open source platform, the Pneumatron represents an easy, low-cost, and powerful tool for field measurements, which can significantly improve our understanding of plant-water relations and the mechanisms behind embolism. (AU)

• Processo FAPESP: 18/09834-5 - Vulnerabilidade ao embolismo em espécies cítricas: características hidráulicas, variação sazonal e relações entre copa e porta-enxerto
• Beneficiário: Marcela Trevenzoli Miranda
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/14075-3 - Novos métodos para estudo das propriedades hidráulicas do xilema e embolismo in vivo em plantas
• Beneficiário: Luciano Pereira
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 18/01847-0 - DendroGrad: aplicando anéis de crescimento, anatomia da madeira e atributos hidráulicos em um gradiente ambiental para avaliar as respostas de crescimento de três espécies tropicais à fertilização por CO2
• Beneficiário: Peter Stoltenborg Groenendyk
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 19/07773-1 - Restaurando ecossistemas neotropicais secos - seria a composição funcional das plantas a chave para o sucesso?
• Beneficiário: Rafael Silva Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Temático