Endo-beta-1,3-glucanase (GH16 Family) from Trichoderma harzianum Participates in Cell Wall Biogenesis but Is Not Essential for Antagonism Against Plant Pathogens

Autor(es):Mostrar menos -

Ribeiro, Marcela Suriani ^[1] ; de Paula, Renato Graciano ^[2] ; Voltan, Aline Raquel ^[1] ; de Castro, Raphaela Georg ^[1] ; Carraro, Claudia Batista ^[3] ; de Assis, Leandro Jose ^[4] ; Steindorff, Andrei Stecca ^[5] ; Goldman, Gustavo Henrique ^[4] ; Silva, Roberto Nascimento ^[3] ; Ulhoa, Cirano Jose ^[1] ; Monteiro, Valdirene Neves ^[6]

Afiliação do(s) autor(es)

^[1] Fed Univ Goias UFG, Biol Sci Inst, Dept Biochem & Cellular Biol, Campus Samambaia, BR-74090900 Goiania, Go - Brazil

^[2] Univ Fed Espirito Santo, Hlth Sci Ctr, Dept Physiol Sci, BR-29047105 Vitoria, ES - Brazil

^[3] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Biochem & Immunol, BR-14049900 Ribeirao Preto - Brazil

^[4] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Ciencias Farmaceut, Av Cafe S-N, BR-14040903 Ribeirao Preto - Brazil

^[5] Univ Brasilia, Biol Sci Inst, Dept Cell Biol, Campus Darcy Ribeiro, BR-70910900 Brasilia, DF - Brazil

^[6] State Univ Goias UEG, Campus Exact Sci & Technol, BR 153, Km 98, BR-75000000 Anapolis, Go - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOMOLECULES; v. 9, n. 12 DEC 2019.
• Citações Web of Science: 2

Resumo

Trichoderma species are known for their ability to produce lytic enzymes, such as exoglucanases, endoglucanases, chitinases, and proteases, which play important roles in cell wall degradation of phytopathogens. beta-glucanases play crucial roles in the morphogenetic-morphological process during the development and differentiation processes in Trichoderma species, which have beta-glucans as the primary components of their cell walls. Despite the importance of glucanases in the mycoparasitism of Trichoderma spp., only a few functional analysis studies have been conducted on glucanases. In the present study, we used a functional genomics approach to investigate the functional role of the gluc31 gene, which encodes an endo-beta-1,3-glucanase belonging to the GH16 family in Trichoderma harzianum ALL42. We demonstrated that the absence of the gluc31 gene did not affect the in vivo mycoparasitism ability of mutant T. harzianum ALL42; however, gluc31 evidently influenced cell wall organization. Polymer measurements and fluorescence microscopy analyses indicated that the lack of the gluc31 gene induced a compensatory response by increasing the production of chitin and glucan polymers on the cell walls of the mutant hyphae. The mutant strain became more resistant to the fungicide benomyl compared to the parental strain. Furthermore, qRT-PCR analysis showed that the absence of gluc31 in T. harzianum resulted in the differential expression of other glycosyl hydrolases belonging to the GH16 family, because of functional redundancy among the glucanases. (AU)

• Processo FAPESP: 16/23233-9 - Caracterização da via de sinalização dependente de cálcio na regulação da expressão e secreção de celulases pelo fungo Trichoderma reesei (Hypocrea jecorina)
• Beneficiário: Renato Graciano de Paula
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 17/04206-3 - Elucidação das vias de sinalização celular mediadas por proteína quinase A (PKA), MAPK e cálcio durante expressão e secreção de celulases pelo fungo Trichoderma reesei
• Beneficiário: Roberto do Nascimento Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular