Exploring the genetic potential of a fosmid metagenomic library from an oil impacted mangrove sediment for metabolism of aromatic compounds

Autor(es)

Pereira de Sousa, Sanderson Tarciso ^[1] ; Cabral, Lucelia ^[1] ; Lacerda-Junior, Gileno Vieira ^[1] ; Noronha, Melline Fontes ^[1] ; Ottoni, Julia Ronzella ^[1] ; Sartoratto, Adilson ^[1] ; de Oliveira, Valeria Maia ^[1]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Res Ctr Chem Biol & Agr CPQBA, Inst Biol, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY; v. 189, FEB 2020.
• Citações Web of Science: 0

Resumo

Aromatic hydrocarbons (AH) are widely distributed in nature, and many of them have been reported as relevant environmental pollutants and valuable carbon sources for different microorganisms. In this work, high-throughput sequencing of a metagenomic fosmid library was carried out to evaluate the functional and taxonomic diversity of genes involved in aromatic compounds degradation in oil-impacted mangrove sediments. In addition, activity-based approach and gas chromatography were used to assess the degradation potential of fosmid clones. Results indicated that AH degradation genes, such as monooxygenases and dioxygenases, were grouped into the following categories: anaerobic degradation of aromatic compounds (20.34%), metabolism of central aromatic intermediates (35.40%) and peripheral pathways for catabolism of aromatic compounds (22.56%). Taxonomic affiliation of genes related to aromatic compounds metabolism revealed the prevalence of the classes Alphaproteobacteria, Actinobacteria, Betaproteobacteria, Gammaproteobacteria and Deltaproteobacteria. Aromatic hydrocarbons (phenol, naphthalene, phenanthrene, pyrene and benzopyrene) were used as the only carbon source to screen clones with degradation potential. Of the 2500 clones tested, 48 showed some respiratory activity in at least one of the five carbon sources used. The hydrocarbon degradation ability of the top ten fosmid clones was confirmed by GC-MS. Further, annotation of assembled metagenomic fragments revealed ORFs corresponding to proteins and functional domains directly or indirectly involved in the aromatic compound metabolism, such as catechol 2,3-dioxygenase and ferredoxin oxidoreductase. Finally, these data suggest that the indigenous mangrove sediment microbiota developed essential mechanisms towards ecosystem remediation of petroleum hydrocarbon impact. (AU)

• Processo FAPESP: 13/22555-4 - Investigação de genes envolvidos na biodegradação de hidrocarbonetos aromáticos a partir do metagenoma de mangue impactado com petróleo
• Beneficiário: Sanderson Tarciso Pereira de Sousa
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/50809-5 - Prospecção de metagenoma microbiano de sedimentos de manguezais na busca por novos compostos bioativos
• Beneficiário: Valeria Maia Merzel
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular