Nonmulberry Silk Based Ink for Fabricating Mechanically Robust Cardiac Patches and Endothelialized Myocardium-on-a-Chip Application

Autor(es)

Mehrotra, Shreya ^{[1, 2]} ; de Melo, Bruna A. G. ^{[3, 1]} ; Hirano, Minoru ^{[1, 4]} ; Keung, Wendy ^{[5, 6]} ; Li, Ronald A. ^{[5, 6]} ; Mandal, Biman B. ^{[2, 7]} ; Shin, Su Ryon ^[1]

Afiliação do(s) autor(es)

^[1] Harvard Med Sch, Brigham & Womens Hosp, Dept Med, Div Engn Med, Cambridge, MA 02139 - USA

^[2] Indian Inst Technol Guwahati, Dept Biosci & Bioengn, Gauhati 781039, Assam - India

^[3] Univ Estadual Campinas, Sch Chem Engn, Dept Engn Mat & Bioproc, BR-13083852 Campinas, SP - Brazil

^[4] Toyota Motor North Amer Inc, Toyota Res Inst North Amer, Future Vehicle Res Dept, 1555 Woodridge Ave, Ann Arbor, MI 48105 - USA

^[5] Univ Hong Kong, Dr Li Dak Sum Res Ctr, Hong Kong - Peoples R China

^[6] Karolinska Inst, Ming Wai Lau Ctr Reparat Med, Hong Kong - Peoples R China

^[7] Indian Inst Technol Guwahati, Ctr Nanotechnol, Gauhati 781039, Assam - India

• Tipo de documento: Artigo Científico
• Fonte: ADVANCED FUNCTIONAL MATERIALS; v. 30, n. 12 JAN 2020.
• Citações Web of Science: 13

Resumo

Bioprinting holds great promise toward engineering functional cardiac tissue constructs for regenerative medicine and as drug test models. However, it is highly limited by the choice of inks that require maintaining a balance between the structure and functional properties associated with the cardiac tissue. In this regard, a novel and mechanically robust biomaterial-ink based on nonmulberry silk fibroin protein is developed. The silk-based ink demonstrates suitable mechanical properties required in terms of elasticity and stiffness (approximate to 40 kPa) for developing clinically relevant cardiac tissue constructs. The ink allows the fabrication of stable anisotropic scaffolds using a dual crosslinking method, which are able to support formation of aligned sarcomeres, high expression of gap junction proteins as connexin-43, and maintain synchronously beating of cardiomyocytes. The printed constructs are found to be nonimmunogenic in vitro and in vivo. Furthermore, delving into an innovative method for fabricating a vascularized myocardial tissue-on-a-chip, the silk-based ink is used as supporting hydrogel for encapsulating human induced pluripotent stem cell derived cardiac spheroids (hiPSC-CSs) and creating perfusable vascularized channels via an embedded bioprinting technique. The ability is confirmed of silk-based supporting hydrogel toward maturation and viability of hiPSC-CSs and endothelial cells, and for applications in evaluating drug toxicity. (AU)

• Processo FAPESP: 17/02913-4 - Microfluídica para cultura 3D de h-AdMSCs em ácido hialurônico livre ou estruturado em esponjas: quimiotaxia e quimiocinese em resposta a gradientes de fatores de crescimento de L-PRP
• Beneficiário: Bruna Alice Gomes de Melo
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado