Core-sheath nanostructured chitosan-based nonwovens as a potential drug delivery system for periodontitis treatment

Autor(es)

dos Santos, Danilo M. ^[1] ; Chagas, Paulo A. M. ^{[1, 2]} ; Leite, Ilaiali S. ^[3] ; Inada, Natalia M. ^[3] ; de Annunzio, Sarah R. ; Fontana, Carla R. ^[4] ; Campana-Filho, Sergio P. ^[5] ; Correa, Daniel S. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Embrapa Instrumentacao, Nanotechnol Natl Lab Agr LNNA, BR-13560970 Sao Carlos, SP - Brazil

^[2] Fed Univ Rio Carlos UFSCar, Ctr Exact Sci & Technol, PPG Biotec, BR-13565905 Sao Carlos, SP - Brazil

^[3] Univ Sao Paulo, Sao Carlos Inst Phys, POB 369, BR-13560970 Sao Carlos - Brazil

^[4] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Clin Anal, BR-14800903 Araraquara, SP - Brazil

^[5] Univ Sao Paulo, Sao Carlos Inst Chem, Ave Trabalhador Sao Carlense 400, BR-13566590 Sao Carlos - Brazil

• Tipo de documento: Artigo Científico
• Fonte: International Journal of Biological Macromolecules; v. 142, p. 521-534, JAN 1 2020.
• Citações Web of Science: 0

Resumo

Core-sheath nanofibers were successfully prepared via coaxial electrospinning by using chitosan with well-defined structural characteristics as the shell layer and poly (vinyl alcohol) (PVA) containing tetracycline hydrochloride (TH) as the core layer. The effects of the average degree of deacetylation ((DD) over bar) of chitosan and the post-electrospinning genipin crosslinking on physicochemical and biological properties of resulting nonwovens were evaluated. Defect-free and geometrically uniform nanofibers with diameters predominantly in the range of 100-300 nm were prepared, and transmission electron microscopy (TEM) revealed the core-sheath structures and its preservation after crosslinking. The mechanical properties, as well as the stability of nonwovens in aqueous medium, were greatly improved by genipin-crosslinking, which enabled a sustained release of TH over 14 days. Results also revealed that the release profile of TH in the presence of lysozyme was affected by the composition of the shell layer, as the TH release rate increases with decreasing of (DD) over bar. Further in vitro antimicrobial activity demonstrated that the cross-linked nonwovens containing TH showed strong activity against bacterial strains associated with periodontal disease. Additionally, the nonwovens did not demonstrate cytotoxic toward fibroblast (HDFn) cells, hence showing their potential for applications as a novel drug delivery platform for periodontitis treatment. (C) 2019 Elsevier B.V. All rights reserved. (AU)

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