Association between Genetic Polymorphisms in RANK, RANKL and OPG and Peri-Implant Diseases in Patients from the Amazon Region

Autor(es)

Raphael Carvalho e Silva ^[1] ; Marilia Bianchini Lemos Reis ^[2] ; Juliana Arid ^[3] ; Elvira Katherine Barriga Flores ^[4] ; Giuseppe Valduga Cruz ^[5] ; Guido A. Marañón-Vásquez ^[6] ; Lizete Karla Filgueiras de Souza ^[7] ; Arthur Belém Novaes Jr ^[8] ; Alexandra Mussolino de Queiroz ^[9] ; Erika Calvano Küchler ^[10]

Afiliação do(s) autor(es)

^[1] Faculdade do Amazonas. Department of Oral and Maxillofacial Surgery and Dental Implants - Brasil

^[2] Universidade de São Paulo. School of Dentistry of Ribeirão Preto. Department of Oral and Maxillofacial Surgery and Periodontology - Brasil

^[3] Universidade de São Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric Dentistry - Brasil

^[4] Universidade Positivo. School of Health Science - Brasil

^[5] Universidade Positivo. School of Health Science - Brasil

^[6] Universidade de São Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric Dentistry - Brasil

^[7] Faculdade do Amazonas. Department of Oral and Maxillofacial Surgery and Dental Implants - Brasil

^[8] Universidade de São Paulo. School of Dentistry of Ribeirão Preto. Department of Oral and Maxillofacial Surgery and Periodontology - Brasil

^[9] Universidade de São Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric Dentistry - Brasil

^[10] Universidade de São Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric Dentistry - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Dental Journal; v. 31, n. 1, p. 63-68, 2020-03-06.

Resumo

Abstract The present study evaluated polymorphisms in RANK, RANKL and OPG-encoding genes to assess whether they are associated with mucositis and peri-implantitis in a population from the Brazilian Amazon region. One hundred and fourteen patients with dental implants were included in the study. After clinical and radiographic examination, the sample was categorized into 4 groups, according to the peri-implant status: Healthy (n=71), Mucositis (n=30), Peri-implantitis (n=13) and Diseased (Mucositis + Peri-implantitis, n=43). Genomic DNA was extracted from buccal cells from saliva, and the genetic polymorphism in osteoprotegerin (OPG), Kappa nuclear factor activator receptor (RANKL) and nuclear kappa factor activator receptor (RANK) were genotyped by the real time PCR. Univariate and multivariate statistical analyses were performed to compare clinical variables among groups and to evaluate genotypes and alleles distributions and the established alpha was 5%. Age, peri-implant biotype, diabetes and presence of peri-implant biofilm were associated with mucositis (p<0.05) and peri-implantitis (p<0.05). Smoking, alcoholism, and periodontal biofilms were also associated with the presence of peri-implantitis (p<0.05). Univariate and multivariate analysis did not demonstrate an association of peri-implantitis or mucositis with any genetic polymorphism in RANK (rs3826620), RANKL (rs9594738) and OPG (rs2073618) (p>0.05). The studied genetic polymorphism in RANK, RANKL and OPG were not associated with mucositis and peri-implantitis in a Brazilian population from the Amazon region. (AU)

• Processo FAPESP: 15/06866-5 - Avaliação do papel do estrógeno no desenvolvimento dentofacial
• Beneficiário: Erika Calvano Kuchler
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores